European Journal of Clinical and Experimental Medicine T.22, z. 1 (2024)

URI dla tej Kolekcjihttps://repozytorium.ur.edu.pl/handle/item/10350

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Assessment of hepatorenal biochemical indices in male Sprague Dawley rats preceding concurrent oral administration of Ghana alcoholic bitters and natural cocoa powder
(Publishing Office of the University of Rzeszow, 2024) Maalman, Raymond Saa-Eru; Bimpong, Samuel; Abaidoo, Chrissie Stansie; Cuba, Magalys Lopez; Kumi, Michael Barima; Sarkodie, Francis Kofi
Introduction and aim. There has been a surge in the consumption of Ghanaian alcoholic bitters. Ghanaian alcoholic bitters are formulated from a maceration of not less than three plant parts making the organic composition very complex. There appear to be no studies on the effect of Ghanaian alcoholic bitters on the hepatorenal biochemistry indices. The study aimed to assess the effects of alcoholic bitters and cocoa powder on the liver and kidney serum biochemistry. Material and methods. Twenty-four healthy male Sprague Dawley rats, age 11–12 weeks, weighing 150–250 g were used. The rats were randomly assigned to four groups (n=6). At the end of the experimentation, a blood sample was taken by cardiac puncture and centrifuged to obtain the serum for biochemical assays and analysis. Results. The liver enzymes showed no significant difference between the treatment and control groups. There were higher mean values for total bilirubin and direct bilirubin for alcoholic bitters and natural cocoa powder groups respectively than the control group and the co-administration of alcoholic bitters and natural cocoa powder group. Conclusion. The study concludes that alcoholic bitters consumption might cause injury to the liver and kidney resulting in anomaly of the hepatorenal indices from rat blood serum biochemistry.
Total resection of foramen magnum meningioma via a far-lateral approach – a case report
(Publishing Office of the University of Rzeszow, 2024-03) Szymoniuk, Michał; Sakw, Leon; Domagalski, Łukasz; Gałązka, Jakub Krzysztof; Hoffman, Zofia; Dryla, Aleksandra; Czyżewski, Wojciech; Szczepanek, Dariusz; Torres, Kamil; Rola, Radosław
Introduction and aim. The foramen magnum is a rare location of meningioma development, accounting for 1.8 to 3.2% of all reported tumors of this type. Microsurgical resection, representing a gold standard in foramen magnum meningioma treat ment, may result in various neurological deficits or incomplete resection due to challenging anatomical conditions. Currently, even surgical resections of foramen magnum meningioma conducted by experienced neurosurgeons are burdened by a relatively high complication rate of 17.2% Description of the case. We report the case of a 25-year-old male who presented increasing headaches and decreasing activity for 5 months. In his medical history, the patient had been diagnosed with cerebral palsy, autism spectrum disorder, and suffered partial seizures. Magnetic resonance imaging revealed anterolateral foramen magnum meningioma. The tumor was resected via a far-lateral approach. After the surgery, the patient maintained a preoperative neurological state without additional neurological deficits. The post-operative magnetic resonance imaging demonstrated complete tumor removal. Histopatholog ical examination revealed transitional meningioma (WHO grade I). Conclusion. Our case demonstrates that the far-lateral approach can be efficient for the resection of anterolateral foramen magnum meningioma. In such cases, Simpson grade 1 can be achieved without complications, providing immediate relief of symptoms and minimizing the risk of recurrence.
The content of lactoferrin and interleukin-8 in breast milk of patients with lactational mastitis
(Publishing Office of the University of Rzeszow, 2024-03) Demianchuk, Nataliia; Akimova, Viorika; Soyka, Larysa; Shchurko, Mariia; Lapovets, Lyubov; Lutsiv, Nataliia; Tkachuk, Serhii; Buchko, Olha; Zubchenko, Svitlana; Hayduchok, Ihor
Introduction and aim. Information concerning lactoferrin and interleukin-8 (IL-8) local levels in breast milk are not numerous and requires further research. The aim of this study was to determine the content of lactoferrin and interleukin-8 in the breast milk of patients with lactational mastitis, and to identify new potential markers for assessing the activity of the inflammatory process in the mammary gland. Material and methods. This study analyzed the breast milk of 30 women with lactostasis (group I), 37 women with lactational mastitis (group II) and 30 healthy lactating women (age 26±5 years old). The milk content of lactoferrin and interleukin-8 (IL-8) was determined by enzyme-linked immunosorbent assay. Results. The average value of lactoferrin in breast milk of healthy women was 4.78±0.47 mg/mL, exceeding levels in group I 1.8 times (p<0.05). The level of lactoferrin in group II exceeds the control values 3.1 times (p<0.05). The content of IL-8 in breast milk of women in group I was 7.3 times higher than the control (3.63±0.12 pg/mL, p<0.05). In lactational mastitis, the concentration of IL-8 in breast milk exceeded the group I 13.9 times (p<0.05) and was 1.9 times higher than group I (p<0.05). Conclusion. The analysis has revealed an increase of lactoferrin and IL-8 in breast milk of the test groups, which indicates the activation of non-specific protection.
Determinants of distress levels in high-risk pregnant women – cross-sectional study
(Publishing Office of the University of Rzeszow, 2024-03) Kara, Pınar; Nazik, Evşen; Çerçer, Zehra
Introduction and aim. Global and national care recommendations indicate that women with high-risk pregnancies should receive personalized and qualified care during this period. This study was conducted to determine the distress levels in high-risk pregnant women and affecting factors. Material and methods. The cross-sectional this study was conducted with total of 416 high-risk pregnant women who met the inclusion criteria in the obstetrics clinic of a training and research hospital. The study data were collected with data collection form and “Tilburg Pregnancy Distress Scale (TPDS).” Results. The mean TPDS total score of high-risk pregnant women was 18.25±6.85. It was found that planning pregnancy, gravida, and diagnosis of gestational hypertension, systemic diseases, and gestational diabetes in the present pregnancy was asso ciated with pregnancy-specific distress (p<0.05; β=0.291, β=0.158, β=0.272, β=0.137, β=0.116, respectively). Conclusion. It is advised that health professionals assess the distress levels of high-risk pregnant women and give personalized care during prenatal period.
Simplified vs modified (reduced) ultrasound 6 joint score in assessing disease activity in rheumatoid arthritis patients
(Publishing Office of the University of Rzeszow, 2024-03) Gabal, Mervat Mamdouh Abo; Eldin, Amina Badr; Elserwy, Maha Ahmed; Mohammed, Mostafa Adel
Introduction and aim. With increasing ultrasound US assessment opportunities for tight rheumatoid arthritis (RA) management, more simplified accurate US-assessment is desired in clinical practice. Aim of the work is assessing modified vs simplified ultrasound 6-joint scores relevance in assessing rheumatoid arthritis disease activity. Material and methods. Fifty-five RA patients were subjected to detailed history, clinical, and musculoskeletal examination with disease activity assessment by clinical disease activity index, simple disease activity index and disease activity score in 28 joints. Com plete blood count, erythrocyte sedimentation rate, C-reactive protein, rheumatoid factor, anti-cyclic citrullinated peptide antibodies were done. Patients underwent US examination (gray-scale and power Doppler) for wrist, 2nd and 3rd metacarpophalangeal and knee joints bilaterally. Synovitis composite score was added. Two US indices were constructed: simplified S6 and modified M6 joint scores. Results. Statistical significant positive correlations were high between S6/M6 score parameters (total, grey-scale (GS), power doppler (PD), Composite) and disease activity markers. Both M6 and S6 scores differentiated mild-moderate and moderate-severe disease activity patients. However, only S6 score differentiated remission from mild disease activity patients. Conclusion. Ultrasound 6-joint scores (especially simplified S6) were rapid, easy and sensitive ultrasound tools assessing rheu matoid arthritis disease activity in clinical practice.
Paroxysmal non-epileptic events vs epilepsy – what we know and where we are in medicine?
(Publishing Office of the University of Rzeszow, 2024-03) Syzdoł, Bartłomiej; Ziółkiewicz, Aleksandra; Sielwanowska, Wiktoria; Żybowska, Monika; Wilczek, Natalia; Szukała,Klaudia; Chrościńska-Krawczyk, Magdalena
Introduction and aim. Paroxysmal non-epileptic events (PNEEs) are neurological conditions that include behavioral changes or disturbances of consciousness. The aim of the article is to compare individual paroxysmal non-epileptic events disorders, to indicate differences in their clinical picture and to discuss their differential diagnosis. Material and methods. A review of the most common non-epileptic paroxysmal events is presented based on the available literature of PubMed and Google Scholar databases from 2000 to 2023. Analysis of the literature. Depending on the age of the child, the nature and type of seizures are variable. Unfortunately, epilepsy is currently overdiagnosed, which results in the inclusion of antiepileptic drugs without the need to use them. This may be related to the immaturity of the central nervous system, malfunctioning of other organs or have a psychogenic background. In most cases, they do not require pharmacological treatment. Conclusion. Paroxysmal non-epileptic events, due to the diverse and uncharacteristic clinical picture, pose a major diagnostic challenge. Because of the current overdiagnosis of epilepsy they should always bear in mind differential diagnosis. This is important because of the differences in the treatment of these disorders.
The influence of therapeutic education on diabetes-related distress and therapeutic adherence among patients with type 2 diabetes in Tetouan, Morocco
(Publishing Office of the University of Rzeszow, 2024-03) Belhaj, Hajar; Barouaca, Hassan; Oudghiri, Dia Eddine; Abderrahmane, Lamiri; Mostakim, Mohammed; Timihiri, Abderrahim; Ahyayauch, Hasna; Younes, Saoud
Introduction and aim. Diabetes-related distress is a prevalent issue that affects many patients, leading to challenges in ad hering to treatment plans and lifestyle modifications. By implementing therapeutic education programs, healthcare providers seek to enhance patients’ understanding of their condition, coping mechanisms, and treatment adherence. This study aims to explore the impact of therapeutic education on diabetes-related distress and therapeutic adherence among individuals diag nosed with type 2 diabetes. Material and methods. This quasi-experimental study involved 40 patients with type 2 diabetes receiving therapeutic educa tion during 6 Months from March 24 to September 30, 2022. Diabetes-related distress and adherence were assessed before and after the intervention using the Diabetes Distress Scale (DDS-17) and glycated hemoglobin (HbA1c) levels. Results. Wilcoxon signed rank tests revealed statistically significant improvements for all measures after the intervention. Patients showed improved DDS-17 scores (p<0.001) across all dimensions, and lower HbA1c levels (p<0.001). Pre- and post-inter vention mean difference across all measures show that the greatest improvements were seen in emotional distress (1.37) and treatment-related distress (1.2). Conclusion. Therapeutic education had a positive impact on therapeutic adherence and diabetes-related distress. It should be an integral part of type 2 diabetes care protocols in Morocco.
Therapeutic advantages of omega-3 fatty acid supplementation in patients with schizophrenia – a systematic review
(Publishing Office of the University of Rzeszow, 2024-03) Vityala, Srilaxmi; Kanteti, Krishna Priya; Vityala, Yethindra; Tagaev, Tugolbai; Damineni, Ujwala
Introduction and aim. In patients with schizophrenia, omega-3 (n-3) polyunsaturated fatty acids (PUFAs) treatment was found to ameliorate the cardiovascular, metabolic, and inflammatory problems caused by antipsychotic medication and even reduce the need for medication by 20%. In this study, we evaluated the potential therapeutic effects of n-3 PUFA supplementation in patients with schizophrenia. Material and methods. The PRISMA guidelines were followed in conducting this systematic review. The Embase, MEDLINE, Web of Science, and Google Scholar databases were searched electronically. The first search yielded 50 papers in total. Subse quently, 43 publications that did not meet our eligibility requirements were removed, and seven articles were selected. Analysis of the literature. The analysis showed that n-3 PUFA supplementation and the placebo group both decreased their psychotic (PANSS and GAF scales) and Calgary Depression Scale symptomatology and boosted their functional ability (GAF) when used as an adjuvant to antipsychotic medication. When administered as a monotherapy with a metabolic antioxidant, n-3 PUFA supplementation proved beneficial for treating schizophrenia. In patients with schizophrenia, n-3 PUFAs have thera peutic benefits as adjuvant treatments to medications, although not for different variables or patient groups. Conclusion. In many studies, patients with chronic schizophrenia who received n-3 PUFA supplementation showed no improvement in their clinical condition.
Expression of vascular endothelial growth factor and microvessel density in oral squamous cell carcinoma and its correlation with various clinico-pathological parameters
(Publishing Office of the University of Rzeszow, 2024-03) Panigrahi, Ranjita; Jha, Narendra Kumar; Hota, Subhransu Kumar
Introduction and aim. Angiogenesis, which is accomplished by capillary sprouting, is the process by which new vessels are created from pre-existing ones. In tumor, once their initial blood supply is depleted, a tumour is unable to grow without additional blood flow. Additionally, a tumor’s microvasculature, or microvessel density (MVD), increases along with its capacity to produce angiogenesis. We aimed to observe the relationship between the expression of vascular endothelial growth factor (VEGF) and MVD (using CD34) in oral squamous cell carcinoma (OSCC). Material and methods. The expression of VEGF and CD34 antibodies was analysed using immunohistochemistry method on 50 cases of histopathologically proved OSCC. The expression was correlated with clinicopathological parameters. Results. A significant correlation was observed between VEGF expression and gender, LVSI. No correlation between any other factors and the difference in VEGF expression was statistically significant. Similarly, the MVD expression was not found to be statistically significant in any of the pathological parameters. Conclusion. VEGF positivity as well as MVD were found to be independent of the tumor pathology. Tumor MVD was found to be independent of the expression of VEGF. Further studies in a larger study group may establish a significant association so that antiangiogenic targeted therapy may be initiated.
Consensus Committee of experts on Kawasaki Disease and Chinese Journal of Contemporary Pediatrics – the expert consensuses on intravenous immunoglobulin, aspirin, and glucocorticoid
(Publishing Office of the University of Rzeszow, 2024-03) Ren, Shuying; Deng, Fangming; Du, Zhongdong; Yang, Xiaodong; Xie, Lijian; Wang, Hong; Jiao, Fuyong
Introduction and aim. Kawasaki disease (KD) is an acute vasculitis with unknown etiology, usually occurring in chil dren under 5 years old. This article will summarize the three consensuses formulated in China about KD. Material and methods. English databases for consensus search include UpToDate, BMJ Clinical Evidence, National Guideline Clearinghouse, Joanna Briggs Institute Library, Cochrane Library, and PubMed, etc.; Chinese databases in clude China Biomedical Literature Service, China Knowledge Network, Wanfang database, etc. All literature searches ended on February 28, 2022. Analysis of the literature. KD is a common acquired heart disease in children and can lead to severe complications such as coronary injury. However, intravenous immunoglobulin (IVIG) combined with oral aspirin (Asp) is currently recognized as the most effective treatment in KD acute stage and the first-line treatment to prevent cardiovascular complications. Glucocorticoid (GC) is mainly used for KD patients with a high risk of coronary artery aneurysm (CAA), no immunoglobulin response, and confirmed CAA. There are already consensus guidelines on diagnosing and treat ing KD in different countries. This article summarizes the relevant expert consensus on aspirin, glucocorticoids and IVIG for the treatment of Kawasaki disease in China. Conclusion. Still, there are inconsistent opinions in the literature on the mechanism, optimal timing, and dosage of medication for KD.
PET and SPECT imaging as a solid guide to detect and discriminate atypical phenotypes of neurodegenerative disorders
(Publishing Office of the University of Rzeszow, 2024-03) Ruffini, Livia; Zilioli, Alessandro; Cervati, Veronica; Lauretan, Fulvio; Misirocchi, Francesco; Maggio, Marcello; Migliari, Silvia; Graziani, Tiziano; Cidda, Carla; Baldari, Giorgio; Spallazzi, Marco; Scarlattei, Maura
Introduction and aim. Atypical or mixed presentations of neurodegenerative disorders may postpone or confound the final diagnosis. Molecular imaging with positron emission tomography (PET) and single photon emission computed tomography (SPECT) radioligands provide target-specific information and may anticipate the diagnosis by “in vivo” detection of the neuro pathological substrate, as Aβ deposition, nigrostriatal dopaminergic depletion or tau inclusions. This concise review will dis cuss the potential of PET and SPECT imaging as a solid guide to better characterize atypical phenotypes of neurodegeneration in the clinical routine, with the potential to drive personalized interventions, improve cohort uniformity for clinical trials, and serve as biomarkers for targeted molecular therapies. Material and methods. Literature search was performed focusing on the role of PET and SPECT imaging in assessing atypical phenotypes of neurodegeneration, using the electronic source of database PubMed/MEDLINE and the web-based search engines Google, Google Scholar. Analysis of the literature. New disease-modifying drugs may increase their effect with early initiation, which is especially im portant in working persons and younger subjects presenting atypical symptoms. In older individuals, the coexistence of neu rodegeneration, age-related changes, cerebrovascular lesions, or depression makes challenging a definitive diagnosis. Quantitative tools able to measure tracer distribution increase the accuracy of molecular neuroimaging creating topographic maps that compare subject’s data with healthy controls databases. Conclusion. Atypical phenotypes may be associated with quantitative key-pattern allowing a more precise and early diagnosis of the neurodegenerative disorder. Finally, quantitative assessment of the pathological substrates allows us to track the disease process and measure treatment response.
Selegiline modulates inflammatory indicators in RAW 264.7 macrophages and LPS-aggravated CFA-induced rheumatoid arthritis in rats
(Publishing Office of the University of Rzeszow, 2024-03) Ahmed, Abdul Baquee; Chetia, Purbajit
Introduction and aim. Rheumatoid arthritis (RA) causes pain, inflammation, and deformities in numerous joints. Monoamine oxidase B (MOA-B) inhibitor selegiline exhibits anti-inflammatory characteristics and has the propensity to scavenge free rad icals. Therefore, the aim of this research comprises of assessing the effect of selegiline on proinflammatory cytokines in RAW 264.7 macrophages as well as its capacity to improve various arthritic parameters in rats with lipopolysaccharide (LPS) acceler ated complete Freund’s adjuvant (CFA) induced RA. Material and methods. In RAW 264.7 cells (lipopolysaccharide accelerated), nitric oxide (NO), tumor necrosis factor alpha (TNF-α), interleukin 6 (IL-6), inducible nitric oxide synthase (iNOS), and prostaglandin E2 (PGE2 ) were determined after treat ment with selegiline. Different arthritic parameters were analyzed after administration of selegiline in LPS accelerated CFA-in duced arthritis in rats. Results. LPS escalates NO, TNF-α, IL-6, iNOS, and PGE2 quantities in the RAW 264.7 cells, which was minimized by selegiline at 100 µg/mL and 150 µg/mL respectively. In rats, CFA induction causes a decrease in body weight, elevation of paw volume, splenic index, and arthritic index, which are further accelerated by LPS. 20 mg/kg of selegiline managed all these arthritic pa rameters effectively, including TNF-α, IL-6, and a few other biochemical parameters. Conclusion. Selegiline may be beneficial in RA extenuating joint and cartilage damage, and modulating inflammatory responses.
Traditional teaching method versus simulation-based teaching method in the prevention of medication errors among nursing students
(Publishing Office of the University of Rzeszow, 2024-03) Ramamurthy, Rohini; Chitra, Felicia; Kavitha
Introduction and aim. The effective and safe administration of medication is the responsibility of every health care provider involved in patient care. One of the biggest problems with nursing education is the gap between theory and practice, which pave ways for medication errors to occur at any level of the medication administration process. This can be rectified by using an efficient teaching strategy that harmoniously blends nursing theory and practical skills. the aim of the study was to evaluate the effectiveness of the traditional teaching method versus simulation-based teaching method on level of knowledge, attitude, and practice on prevention of medication errors among nursing students in selected colleges, Puducherry. Material and methods. The research approach and design used for the study were quantitative approach and quasi-experi mental pre and post-test control group design respectively. The study settings were selected 4 nursing colleges in Puducherry. The sample size was 100 (50 in experimental group I and 50 in experimental group II) which was selected by using simple ran dom and stratified sampling technique. pre-test was done to assess the level of knowledge, attitude and practice on preven tion of medication error for both experimental groups utilizing the self-administered knowledge, attitude questionnaire and checklist. The experimental group I received traditional teaching method and experimental group II received simulation-based teaching method. Post-test was done after one week using the same tool. Results. The study results revealed that out of 100 nursing students, majority 35 (70%) and 38 (76%) of the nursing students were in the age group of 20–21 years, 40 (80%) and 39 (78%) of them were female, 50 (100%) and 41 (82%) of them were staying as day scholars, 40 (80%) and 41 (82%) had one attempt to clear the pharmacology subject in the experimental group I and II respectively. The level of knowledge, attitude, practice showed a statistically significance difference at p<0.05 between the pre and post-test within the experimental group I and II respectively. The effective mean scores of knowledge, attitude and practice showed a statistically significance difference at p<0.05 between the experimental group I and II respectively, revealed that the simulation-based teaching method was more effective over the traditional teaching method. There was a significant positive correlation at p<0.05 exist between the level of knowledge and attitude, knowledge and practice in the experimental group I. The association between the post-test level of attitude and the gender variable showed a statistically significant at p<0.05 in the experimental group II. Conclusion. The study concluded that the simulation-based teaching method was effective which can be utilized as a means to educate the nursing students during their academic performance.
Assessment of serum creatinine, urea, and aminotransferase levels among methamphetamine addicted individuals in Khartoum State
(Publishing Office of the University of Rzeszow, 2024-03) Al-Tayeb, Al-Noor Opied; Abubakr, Mohammed Hayati; Elrheima, Hafsa Ahmed; Habbani, Ahmed Khalid; Elrasul, Rami Y. Hassab
Introduction and aim. Methamphetamine-use disorder is a pressing global public health issue. In Sudan, the escalating meth amphetamine (METH) consumption has become a significant social and health problem. This study aims to evaluate liver and kidney biomarkers in methamphetamine addicts in Khartoum state Material and methods. The study was an analytical prospective cross-sectional hospital-based study. One hundred partici pants were enrolled in this study, fifty were cases (methamphetamine addicts), and others were healthy non-METH users as a comparative group. Results. METH users had a mean age of (27±7) years and had been using METH for an average of (14±9) months. Urea and cre atinine levels were also significantly elevated in METH users compared to non-users, with p<0.001 and p=0.044, respectively. Their aspartate transaminase (AST) and alanine transaminase (ALT) levels were significantly higher compared to non-users, with p<0.001. Conclusion. There was significant increases in creatinine, urea, and aminotransferases levels in the case group. ALT showed a moderate positive correlation with abuse duration, while AST showed no significant correlation. Urea and creatinine levels had strong and moderate positive correlations with abuse duration, respectively.
Comparison of algorithms for detection of active inflammatory lesions in sacroiliitis
(Publishing Office of the University of Rzeszow, 2024-03) Gawłowski, Igor; Ożga, Joanna; Raczko, Agata
Introduction and aim. Artificial intelligence is increasingly being used in the medicine, particularly in radiological diagnosis of diseases such as an axial spondyloarthritis (axSpA). The aim of this study is to compare the available algorithms designed to detect active sacroiliitis in patients with axSpA. Material and methods. Four algorithms, two semi-automated and two full-automated for the assessment of bone marrow ede ma (BME) on magnetic resonance imaging (MRI) of the sacroiliac joints (SIJs) were included in the study. They were described and compared in terms of specificity, sensitivity, and correlation of BME detection findings between AI and experts. Analysis of the literature. Among all automated algorithms, the one created by Bressem et al. had the highest number of ex aminations analyzed in the study, involving 593 MRIs of SIJs. The sensitivity and specificity, as well as the correlation between the AI’s detection of BME versus manual, were not calculated for each algorithm. Rzecki’s algorithm had the greatest sensitivity and specificity for BME detection reaching 0.95 and 0.96, respectively. In addition, its Speraman’s coefficient of correlation be tween manual and automated measurements was 0.866. Conclusion. Each of described algorithms is certainly useful in assessing BME in the MRI examinations of SIJs.
Hemodialysis dose and long-term COVID-19 outcomes – a retrospective cohort study
(Publishing Office of the University of Rzeszow, 2024-03) Stepanova, Natalia; Rysyev, Andriy; Snisar, Lyudmyla
Introduction and aim. Long-term outcomes of COVID-19 pose a global challenge, particularly impacting individuals with un derlying health conditions, including those who have undergone hemodialysis (HD). The study aimed to investigate the rela tionship between preexisting dialysis dose, measured by single pool Kt/V (spKt/V), and long-term outcomes of COVID-19 in patients undergoing HD. Material and methods. Demographic, clinical, and laboratory parameters following COVID-19 recovery, and long-term out comes, including the presence of COVID-19 sequelae, hospitalization, and all-cause mortality during a year after COVID-19 were retrospectively analyzed. Results. Out of the 195 patients included, there were 108 males (55.4%) and 87 females (44.6%), with a median age of 56 (44-63) years and a dialysis duration of 49 (31.3–85.2) months. Patients with spKt/V<1.4 had a significantly increased risk of long-term COVID-19 sequelae (HR 9.1, 95% CI: 3.4; 24.6), hospitalization (HR 7.6, 95% CI: 3.9; 14.6), and all-cause mortality (HR 8.5, 95% CI: 2.9; 25.8) within one year after COVID-19 recovery compared with those with spKt/V≥1.4. spKt/V cutoff point of ≤1.3 emerged as a significant risk factor for one-year hospitalization and mortality within our cohort. Conclusion. Suboptimal dialysis dose, as indicated by spKt/V < 1.4, is associated with adverse long-term COVID-19 outcomes in patients undergoing HD. Optimizing dialysis adequacy may mitigate these risks. Further research is needed to validate these f indings and explore interventions to improve outcomes in this vulnerable population.
Exploring barriers to vasectomy adoption among married men in Dadra and Nagar Haveli
(Publishing Office of the University of Rzeszow, 2024-03) Patel, Ronak Dhirubhai; Dabhelker, Monika M.; Patel, Priya Mahendrabhai; Solanki, Preeti
Introduction and aim. Vasectomy was introduced in India in 1992 and is the most effective, simple, and safe permanent method of contraception yet its use is very limited among the population. The study’s objectives were to assess the knowledge, attitude, and perception towards vasectomy and the barriers to adoption among married men in Dadra and Nagar Haveli. Material and methods. A cross-sectional hospital-based study was conducted on married male attendants of patients and data were collected on sociodemographic details, knowledge regarding vasectomy, their attitude and perceptions towards vasec tomy, and their intention of using vasectomy in the future. Results. Out of 300 participants, 258 (86%) participants have heard of vasectomy as a contraceptive method, and only one participant has undergone vasectomy. The majority (285; 95%) of the participants agreed that family planning is also a responsi bility of males while nearly two-third (185; 61.7%) of them were not willing to undergo vasectomy in the future. Procedure-re lated factors, post-procedure-related factors, social factors, availability of alternate contraceptive methods, and preference for tubectomy were the barriers to the adoption of vasectomy. Conclusion. There is a need to provide correct information and counseling about vasectomy and non-scalpel vasectomy to eligible couples to increase the acceptance of vasectomy as a safe, effective, and cheaper method of contraception.
Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction
(Publishing Office of the University of Rzeszow, 2024-03) Manivannan, Kavitha; Ramasamy, Malathi; Sundaresan, Uma; Moustafa, Samar M.; Sherloumay; Mariyam, Safna
Introduction and aim. Brucellosis is a zoonotic disease. Experimental clinical and laboratory diagnosis is still facing problems in identifying the organism. The present study will diagnose a Brucella infection in camel blood in Qatar using serological assays. Isolation and identification were performed on a camel blood sample. Brucella in bacterial isolates was determined by real-time polymerase chain reaction (RT-PCR) as a gold standard test. Material and methods. A total of 220 samples, 200 random serum samples, and 20 EDTA blood samples were selected among the above-mentioned random samples, and 20 serum samples from camel handlers were collected from Al Shahaniya prov ince, Qatar. The Rose Bengal test (RBT), buffered antigen plate agglutination test (BAPAT), and enzyme linked immunosorbent assay (cELISA) for the monoclonal antibody in serum samples were performed using commercially available kits. For the molecular detection of Brucella, conventional PCR and real-time PCR (GPS kit) were used for the genus-specific insertion sequence IS711. Brucella melitensis (MICROBOSS Hightech GmbH kit) was used to identify subspecies. Results. The results identified by vitek2 compact (30%) showed B. melitensis in 6 samples out of 20 isolates. Both conventional (66.67%) and RT-PCR (83.33%) analyses supported this, demonstrating the presence of Brucella. These tests also showed that Brucella species were present in Rose Bengal 182/200 (91%), BAPAT 182/200 (91%), and cELISA (90%) 180/200 in camel serum. Conclusion. To conclude, the prevalence of brucellosis in dromedary camels is higher in this region, and as a matter of urgency, measures should be taken to control the disease.
Patterns and characteristics of traumatic dental injuries in children – a retrospective study in a dental hospital in Kolkata, India
(Publishing Office of the University of Rzeszow, 2024-03) Goswami, Suchetana
Introduction and aim. Traumatic dental injuries (TDIs) in children can have aesthetic, functional, and psychological conse quences. This retrospective study aimed to investigate the epidemiology and patterns of TDIs in a hospital-based population. Material and methods. Patient records from the Department of pediatric and Preventive Dentistry in a dental college and hospital in Kolkata, India, were reviewed. A total of 381 patients, aged 1 to 12 years, who presented one or more traumatized an terior teeth between September 2018 and August 2022, were included. Data on age, sex, mechanism of injury, type of trauma, dentition type, and number of affected teeth were collected. The World Health Organization classification system was used to classify the type of dental trauma. Results. Among the study population, there were 235 males (61.7%) and 146 females (38.3%), with a mean age of 7.67±3.87 years. Falls were the most common cause of trauma (52.2%). The maxillary central incisors were the most frequently affected teeth in both primary and permanent dentitions. Conclusion. Falls were the most common cause of trauma and the maxillary central incisors were the most commonly affected teeth in children.
Antibacterial, DNA photocleavage and molecular docking studies of newly prepared Schiff-based macrocyclic complexes
(Publishing Office of the University of Rzeszow, 2024-03) Mishra, Purti; Sethi, Pooja; Ramasamy, Selva Kumar; Saini, Adesh K.; Tuli, Hardeep Singh; Mittal, Divya; Trehan, Aarti
Introduction and aim. At present, several microbial diseases are prominent and of concern worldwide. The intent of this study was to examine the antibacterial potential of newly synthesized tetradentate macrocyclic complexes against different bacte rial strains. The macrocyclic scaffold has gained attention as a biologically active class of supramolecular chemistry due to its unique properties and ability to target various microorganisms. Thus, the goal of the present study was to develop a series of biologically active transition metal-based macrocycles. Material and methods. All macrocyclic compounds were synthesized by a template method and validated by molar conductiv ity, elemental studies, and spectral and magnetic studies. Antibacterial activities of all metal complexes were evaluated against Escherichia coli (MTCC 739) and Staphylococcus aureus (MTCC 731) bacterial strains by taking ampicillin as a standard reference drug. DNA photocleavage potential was explored using agarose gel electrophoresis. Results. Results revealed the formation of novel macrocyclic complexes via tetra nitrogen bond trapping of metals. Copper complexes have strong potential against S. aureus bacteria as copper and nickel both show good DNA photocleavage potential. Conclusion. The findings endorse the biomedical relevance of these macrocyclic scaffolds, suggesting avenues for further exploration in targeted drug delivery and potential clinical applications. The proposed octahedral geometry for the complexes enhances our understanding of their structural aspects. This research contributes substantively to the field, laying the foundation for future investigations in advanced antimicrobial design and application.

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Przeglądanie European Journal of Clinical and Experimental Medicine T.22, z. 1 (2024) według Data wydania