Introduction and aim. Methamphetamine-use disorder is a pressing global public health issue. In Sudan, the escalating meth amphetamine (METH) consumption has become a significant social and health problem. This study aims to evaluate liver and kidney biomarkers in methamphetamine addicts in Khartoum state Material and methods. The study was an analytical prospective cross-sectional hospital-based study. One hundred partici pants were enrolled in this study, fifty were cases (methamphetamine addicts), and others were healthy non-METH users as a comparative group. Results. METH users had a mean age of (27±7) years and had been using METH for an average of (14±9) months. Urea and cre atinine levels were also significantly elevated in METH users compared to non-users, with p<0.001 and p=0.044, respectively. Their aspartate transaminase (AST) and alanine transaminase (ALT) levels were significantly higher compared to non-users, with p<0.001. Conclusion. There was significant increases in creatinine, urea, and aminotransferases levels in the case group. ALT showed a moderate positive correlation with abuse duration, while AST showed no significant correlation. Urea and creatinine levels had strong and moderate positive correlations with abuse duration, respectively.