European Journal of Clinical and Experimental Medicine

URI dla tego Zbioruhttp://repozytorium.ur.edu.pl/handle/item/2798

European Journal of Clinical and Experimental Medicine (poprzednio: Przegląd Medyczny Uniwersytetu Rzeszowskiego i Narodowego Instytutu Leków w Warszawie; Medical Review) to recenzowane czasopismo (kwartalnik), wydawane przez Uniwersytet Rzeszowski. Do trzeciego kwartału 2015 roku wersję pierwotną czasopisma stanowiło wydanie papierowe. Od czwartego kwartału 2015 roku wersją pierwotną (referencyjną) czasopisma jest wydanie elektroniczne. European Journal of Clinical and Experimental Medicine jest wydawane w wersji angielskiej. Tematyka prac publikowanych w czasopiśmie obejmuje zagadnienia związane z szeroko pojętą medycyną, medycyną kliniczną, doświadczalną, rehabilitacją, fizjoterapią, farmakoterapią, a także tematy dotyczące oceny jakości leków i bezpieczeństwa ich stosowania. Redakcja publikuje również przedruki (standardy, zalecenia, czy opinie Konsultantów Krajowych różnych dziedzin medycyny) po uprzednim uzyskaniu zgody wydawcy.

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19F MRI As a tool for imaging drug delivery to tissue and individual cells
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2017) Bober, Zuzanna; Aebisher, David; Ożóg, Łukasz; Tabarkiewicz, Jacek; Tutka, Piotr; Bartusik-Aebisher, Dorota
Over the past few decades, magnetic resonance imaging (MRI) has proven to be extremely successful in medical applications. More recently, the biomedical applications of MRI have been gaining more use in the field of clinical pharmacy. In 1977, perfluorocarbon compounds (PFC), which form emulsions that can carry drugs, were analyzed by 19F MRI and emulsified PFC compounds have been investigated as potential blood substitutes since the early 1960s and now a wide variety of PFC compounds are currently available as 19F MRI biomarkers. Molecules with 19F substituents are particularly attractive for use in drug tracking by 19F MRI due to 100% 19F abundance, high 19F MRI sensitivity (0.83 relative to 1H MRI) and an impressively large chemical shift range (400 ppm). Another benefit in the use of 19F MRI is a zero background signal in biological samples due to lack of endogenous fluorine. Therefore, drugs containing fluorine atom have potential for 19F MRI imaging drug delivery to tissue. This article will review recent developments in the use of 19F MRI in imaging drug delivery to tissue and individual cells.
A 16-year-old patient with Charcot Marie Tooth disease in variant c.217G>C of the INF2 gene and focal glomerulosclerosis – a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Przygoda, Maria; Matias, Dawid; Jurczak, Maciej; Sokołowska, Aldona; Raba, Karolina; Wołkanowski, Juliusz; Rydzanicz, Małgorzata; Kosińska, Joanna; Płoski, Rafał; Aebisher, David; Pyrkosz, Antoni
Introduction. Charcot Marie Tooth disease (CMT) is currently one of the most commonly diagnosed and commonly hereditary sensorimotor neuropathies. Concluding from the literature, this is the first study describing the case of a patient with CMT disease in the c.217G> C variant of the INF2 gene and focal segmental glomerulosclerosis. Aim. To present a case of a 16-year-old patient suffering from CMT disease in variant c.217G> C of the INF2 gene and focal glomerulosclerosis. Description of the case. The text describes the CMT disease in a patient who underwent the WES / WGS-NGS genetic test and found a mutation within the INF2 gene at the chromosomal position hg38 14: 104701582-G> C, cDNA level c.217 G> C , notation at the p protein level (Gly73Arg). Genotype record according to Human Genome Variation Society: NM_022489.4: c. [217G> C]; [217 =]. The publication includes data on genetics, molecular mechanisms of the disease, diagnostic methods, rehabilitation and surgical treatment. Conclusion. CMT disease is a heterogeneous group of diseases caused by mutations in various genes. The incidence of this pathology has increased significantly in the last century. Currently, there are no treatments available to combat this disease, and symptomatic treatment is the only treatment available.
A benign entity – cerebral multinodular and vacuolating neuronal tumor
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2022) Kış, Naciye; Erok, Berrin; Kılıç, Harun; Önder, Hakan
Introduction and aim. Multinodular and vacuolating neuronal tumor (MVNT) of the cerebrum is a rare benign, mixed glial/ neuronal lesion which has been included in the recent (2016) World Health Organization (WHO) Classification of the central nervous system tumors.Most of the reported cases are remarkable with adult onset seizure in the literature.They can also be found incidentally in nonepilepsy patients with or without headache We aimed to present this unique entity with its typical magnetic resonance imaging (MRI) features. Description of the case. A 21-year old man presented with complaint of headache that increased in frequency within the last few months.No relevant seizure or any other signs of note.He was diagnosed with MVNT by imaging andstarted to be followed- up.The repeat MRI 6 months later showed no interval changes. Conclusion. Clinicians should be aware of that it is a do not touch lesion in asymptomatic patients with no need for biopsy or surgery and follow up imaging is sufficient when presented with the typical MRI manifestations. Surgical resection may be required for seizure control and was reported in few cases with no tumoral regrowth in the literature.
A comparative analysis of the rehabilitation course of two patients after the implantation of an artificial hip joint
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2018) Handkiewicz, Krzysztof
Introduction. Endoprosthetics is the most effective method of returning to a normal way of life without pain. The key element is the post-operative rehabilitation program where a patient has to comply with certain rules and principles. The rehabilitation also includes the family of the patient. Each person, after any surgery requires an individual approach, and no patient can be commonly treated. Aim. The aim of the article is to compare the effects of an early rehabilitation after the implantation of an artificial hip joint in two patient cases. Description of the cases. This research was conducted at Bodden-Klinken GmbH. An individual case study was used, based on an example of two patients at the age of 59 who had implantation of an artificial hip joint. The carried out rehabilitation of the patients was conducted without any disruptions. Patients were able to freely walk on crutches nine days after the procedure. Conclusions. In the cases analyzed, the rehabilitation course was described until the ninth day after implantation of an artificial hip joint. High results in terms of patient rehabilitation were achieved. Furthermore, in order to continue their therapy, they were referred to the sanatorium.
A concurrent outbreak of COVID-19 and methanol poisoning in Iran: Is this the time to make amendments to alcohol drinking laws?
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2020) Banagozar Mohammadi, Ali; Vahabzadeh, Maryam
A cross-sectional study on knowledge, attitude, and practice among type 2 diabetes mellitus patients attending a primary health care center in the rural region of Tamil Nadu
(Publishing Office of the University of Rzeszow, 2024-09) Williams, Helen; Ranganathan, Srinivasan
Introduction and aim. Diabetes mellitus (DM) is a basic metabolic disease of inadequate control of blood glucose levels. Hyperglycemia is exacerbated, and type 2 diabetes mellitus (T2DM) progresses both insulin resistance and β-cell dysfunction. Management of T2DM involves both lifestyle modification and pharmacological therapy. To achieve optimized health outcomes, the patient requires adequate knowledge, attitude, and practice, so educating the patients on these diseases is an effective strategy to reduce complications of T2DM. Material and methods. This is a cross-sectional study which was conducted from August 2022 to January 2023. A total of 200 participants were enrolled with inclusion criteria to determine the knowledge, attitude, and practice of type 2 diabetes mellitus patients. Results. The mean knowledge, attitude and practice (KAP) was 71.5%, 87.5%, and 40% respectively. There is a significant association between knowledge, attitude, practice questions, and socio-demographic characteristics. Education was strongly associated with having higher knowledge scores (p=0.001). Conclusion. The study’s conclusions made clear the necessity of well-planned interventions to raise T2DM awareness among patients with low levels of education. Patients with T2DM may benefit from well-designed educational programs that encourage healthy behavior and these interventions can improve the quality of life of patients in rural region of Tamil Nadu.
A family screening of CD19 gene mutation by PCR-RFLP
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2022) Karaselek, Mehmet Ali; Kapaklı, Hasan; Güner, Şükrü Nail; Kurar, Ercan; Küççüktürk, Serkan; Keleş, Sevgi; Reisli, İsmail
Introduction and aim. Mutation(s) in the gene encoding the CD19 molecule affect CD19 protein expression and primary immunodeficiency (PID) occurs. The PCR-RFLP method, which is faster and cheaper than other mutation detection methods, is rarely used in the diagnosis of PID. The study aimed to genetically identify CD19 deficiency, which is a PID, using the PCR-RFLP method. Material and methods. A total of 8 patients and two healthy controls were included in the study and the relevant region genotypes in the CD19 gene were determined by performing PCR-RFLP analysis. Results. The index case, newborn baby and mother were also included in the study. It was determined that the index case (P6) was homozygous mutant, the newborn baby (P7) and mother (P8) had heterozygous genotype. Based on this situation, one child (P1) was found to be homozygous mutant, mother (P2), father (P3) and other children (P4 and P5) had heterozygous genotype in the family, which was determined to be related to the first case. Conclusion. In our study, it has been shown that PCR-RFLP is a method that can be used in the diagnosis of PID by determining genotypes using PCR-RFLP, and especially in terms of rapid genetic testing of family screenings.
A long clinical course with late distant metastases from follicular thyroid carcinoma
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Erok, Berrin; Harmancı, Kemal; Aksaray, Ferdi; Unlu, Nazmi Uğur; Aydın, Seckin
Introduction. Follicular thyroid carcinoma (FTC) accounts for 10-20% of the differentiated thyroid carcinomas (DTCs), and it is the second most common thyroid malignancy after papillary thyroid carcinoma (PTC). FTC is typically more common in women and in older age group than PTC. Unlike PTC, FTC metastases late to the lymph nodes, with only up to 10-20% of the patients having nodal metastases at the time of diagnosis.On the other hand, distant metastasis via hematogenous spread is more likely in patients with FTC due to the invasion of blood vessels. Prognosis depends on the extent of the distant metastasis which drop 10-year survival significantly. Aim. Although DTCs have usually favorable prognosis, metastatic disease in these patients has a long clinical course. Cranial imaging in these patients should be performed during the follow-up after the treatment of FTC with thyroidectomy and RAI. Description of the case. We report a case of late onset but catastrophic hematogenous distant metastases beginning 8 years after the diagnosis & treatment of FTC and becommimg widespread during the following 10 years with a long clinical course in a 60 year old female patient. Conclusion. In the RAI refractory metastatic lesions SRS and surgical resections should be conserned as the first management approach to improve survival.
A new local therapy of periodontitis in the course of stomach pathology and tobacco smoke intoxication
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Romanova, Iuliia; Zolotukhina, Olena; Shnaider, Stanislav; Kravchenko, Lyudmila; Noneva, Nataliia
Introduction. Inflammatory periodontal diseases, arising against a background of stomach pathology from tobacco addiction remain an acute problem of modern dentistry. Aim. The experimental assessment of a new local treatment efficiency during therapy of simulated periodontitis with hyperacid gastritis and the tobacco smoke intoxication. Material and methods. The work was conducted in 2 stages. At the first stage, all experimental animals were divided into 4 groups: І — intact, II — with simulated periodontitis, III — with simulated periodontitis and hyperacid gastritis, IV — with simulated periodontitis with hyperacid gastritis and tobacco smoking. The local therapy efficiency was evaluated with the use of a new preparation for oral care and a comparison product conducted at the 2nd stage in rats with simulated periodontitis with hyperacid gastritis and tobacco smoking. Results. Experimental periodontitis with hyperacid gastritis and tobacco smoking provokes considerable changes in the periodontal tissues typical for the inflammatory process: lipid peroxidation activity rises and antioxidant system activity reduces. A local therapy in rats resulted in correction of detected metabolic disorders, improving removal of the damaging factors harmful influence and restoring the periodontal tissues condition. Conclusion. The medical efficiency of a new gel normalizes the influence of lipid peroxidation processes, inflammation and the oral cavity protective system activation during periodontitis which arises up against a background of the concomitant pathology of stomach – hyperacid gastritis.
A novel method of immunomodulation of endothelial cells using Streptococcus pyogenes and its lysate
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Arokiaraj, Mark Christopher; Wilson, Jarad
Introduction. Coronary artery diseases and autoimmune disorders are common in clinical practice. Aim. In this study, Streptococcus pyogenes and its lysate were studied to modify the endothelial function. Material and methods. HUVEC cells were seeded in the cell culture, and Streptococcus pyogenes were added to the cell culture, and the supernatant was studied for the secreted proteins. In the second phase, the bacterial lysate was synthesized, and the lysate was added to cell culture and studied. Results. When S. pyogenes alone was added to culture, E Cadherin, Angiostatin, EpCAM and PDGF-AB were some of the biomarkers elevated significantly. HCC1, IGFBP2 and TIMP were some of the biomarkers which showed a reduction. When the lysate was added, the cell-culture was maintained for a longer time, and it showed the synthesis of immune regulatory cytokines. Heatmap analysis showed a significant number of proteins/cytokines concerning the immune/pathways, and toll-like receptors superfamily were modified. BLC, IL 17, BMP 7, PARC, Contactin2, IL 10 Rb, NAP 2 (CXCL 7), Eotaxin 2 were maximally increased. By principal component analysis, the results observed were significant. Conclusion. There is potential for a novel method of immunomodulation of the endothelial cells, which have pleiotropic functions, using S. pyogenes and its lysates.
A patient with overlap syndrome: systemic lupus erythematosus, dermatomyositis, and Sjögren’s syndrome – a rare overlapping diseases case report
(Publishing Office of the University of Rzeszow, 2023-09) Sokołowska, Aldona; Iwański, Mateusz; Dąbrowski, Piotr
Introduction and aim. Autoimmune rheumatic diseases are a group of disorders with similar clinical, laboratory and immunological manifestations. Connective tissue diseases include systemic scleroderma, dermatomyositis or polymyositis, Sjögren’s syndrome, rheumatoid arthritis, and systemic lupus erythematosus. If the patient meets the diagnostic criteria for at least two of these diseases and has specific serologic markers, a diagnosis of overlap syndrome is possible. Description of the case. This case describes a 27-year-old man who had a history of paroxysmal fever, night sweats, erythema-like skin lesions on the forearms and lower legs, a feeling of progressive muscle weakness especially in the proximal muscles, and dry mouth. The patient was diagnosed with an overlap syndrome: systemic lupus erythematosus, dermatomyositis, and Sjögren’s syndrome. Conclusion. Overlap syndrome is difficult to treat due to its multisystem nature, requiring a symptomatic therapeutic approach and careful control of medication doses to reduce side effects while controlling disease activity.
A potential usage of probiotics in prevention and treatment of neutropenic enterocolitis
(Publishing Office of the University of Rzeszow, 2023-03) Gałązka, Jakub Krzysztof; Homa, Piotr; Domagalski, Łukasz
Introduction and aim. Neutropenic enterocolitis is a severe disease that affects patients with immunodeficiency and is often related with the chemotherapy of the hematologic malignancies. Its pathophysiology is not fully understood. Neutropenic colitis may lead to sepsis, gastrointestinal bleeding and even perforations requiring surgical management. The therapy consists of antibiotic therapy, transfusions, hematopoietic growth factor treatment, usage of fluids and electrolytes, bowel rest and even surgical operations. The aim of this review is to consider the potential usage of probiotics in the prevention and treatment of neutropenic enterocolitis. Material and methods. References for that article were found through PubMed and Google Scholar, using terms: “neutropenic enterocolitis” and “probiotics”, or “gut microbiota” and “neutropenic enterocolitis”. The research was limited to abstracts and available full-text articles. Analysis of the literature. The most possible mechanism of neutropenic enterocolitis development appoints bacterial invasion with co-existing immunodeficiency. The probiotics appeal as beneficial agents in both prevention and treatment of neutropenic enterocolitis in according to their impact on gut immune barrier improvement. However older societies’ guidelines were cautious, the most modern ones appoint probiotics as a promising agent in neutropenic enterocolitis, what corresponds with results from current randomized clinical trials. Conclusion. As neutropenic enterocolitis is a severe disease we need to look for better or alternative therapies of that state. The probiotics seems to have beneficial effects in terms of prevention and treatment of neutropenic enterocolitis due to their impact on gut immune barrier. Benefits of such therapy are reflected in current societies’ guidelines which consider probiotics as a promising agent in neutropenic enterocolitis.
A promising approach to wound healing – in-vivo study of carbon nanodots infused PVA hydrogel with Kamias extract as antibacterial wound dressing
(Publishing Office of the University of Rzeszow, 2023-06) Hipolito, Marilene C.
Introduction and aim. The use of carbon nanodots (C-nanodots) synthesized from Kamias leaves for developing antibacterial wound dressings has gained attention due to their potential in promoting wound healing and contraction. To extract Kamias leaves, synthesize C-nanodots through microwave-assisted pyrolysis, characterize the synthesized C-nanodots, and test the polyvinyl alcohol (PVA) hydrogel infused with C-nanodots for antibacterial activity and wound contraction in Sprague Dawley rats. Material and methods. Kamias leaves extract was used to synthesize C-nanodots with varying amounts of monoethanolamine. The C-nanodots were characterized using UV-Vis spectrophotometer, electron microscope, and the paper disk method. The PVA hydrogel infused with C-nanodots was tested for antibacterial activity and wound contraction in Sprague Dawley rats. Results. The synthesized C-nanodots exhibited antibacterial properties against Staphylococcus aureus and Subtilis bacillus, with a zone of inhibition ranging from 15 mm to 23.6 mm at different concentrations. The carbon nanodots-PVA hydrogel patch showed potential wound healing ability, with significant differences in wound contraction compared to the positive and negative controls. Conclusion. C-nanodots synthesized from Kamias extract have potential applications in antibacterial and wound healing fields. However, further studies are required to investigate the mechanism of action and potential side effects of using carbon nanodots in these applications.
A prospective, interventional clinical study to evaluate the safety and efficacy of Liv.52 DS in the management of non-alcoholic fatty liver disease
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2015) Siregar, Gontar; Paramesh, Rangesh; Kumawat, Rajesh; Palaniyamma, D; Srikrishna, HA
Introduction. Non-alcoholic fatty liver disease (NAFLD) is excessive fat build-up in the liver due to causes other than alcohol use. Aim. To evaluate the clinical efficacy and safety of Liv.52 DS tablets in the management of NAFLD. Material and methods. Prospective, interventional clinical study conducted on 60 patients of both sex, aged between 18-65 years, confirmed with NAFLD from clinical examination, laboratory test, ultrasound findings and those willing to give informed consent. All patients received Liv.52 DS at a dose of 2 tablets twice daily for 2 months. All patients were evaluated at baseline, end of 1st month, and end of 2nd month for liver function tests, hepatomegaly by ultrasound, NAFLD Fibrosis Score, lipid profile, hematology and biochemical investigations. Results. Study data was analyzed with GraphPad Prism Software Version 6.07. Data of those patients who completed the study was considered for analysis. Significant improvement in hepatomegaly, liver enzymes was observed. NAFLD fibrosis score revealed no progression of liver fibrosis due to NAFLD during the study period. No abnormal lab values were recorded and there were no adverse events reported during the study. Conclusion. Study concludes that Liv.52 DS is safe and beneficial in individuals suffering from NAFLD.
A rare and overlooked cause of massive gastrointestinal bleeding: Distal duodenal GIST
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Düzenli, Tolga; Köseoğlu, Hüseyin; Erenler, Behice Hande
Introduction. Gastrointestinal stromal tumors (GIST) are tumors of mesenchymal origin which originate from the walls of gastrointestinal system (GIS) organs. Aim. In this case report we aim to discuss the clinical, labaratory and radiological presentation of distal duodenal GIST as a rare and overlooked cause of life-threatining GIS bleeding. Description of the case. A 76-year-old male patient was presented to the emergency department with massive gastrointestinal bleeding. Computerized tomography revealed a mass soft tissue density of 4x4cm at the level of the 3-4th segment of the duodenum. At the endoscopy, there was a deep ulcer in the proximal part of the 3rd segment of the duodenum with a diameter of 2 cm with a bleeding vessel protruding into the lumen. After endoscopic treatments, biopsies were taken from the edges of the ulcer. Histopathological examination revealed a sheet-like infiltration composed of mildly pleomorphic cells with oval-spindle nuclei and abundant eosinophilic cytoplasm in the duodenal lamina propria, as the patient was diagnosed of GIST. Conclusion. GIST and its clinical, labaratory and radiological presentation should be kept in mind in the approach to massive duodenal GIS bleeding.
A renal abscess in the isthmus of horseshoe kidney
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2017) Balawender, Krzysztof; Orkisz, Stanisław; Biela, Przemysław; Sęk-Mastej, Anna
Horseshoe kidney (HSK) is the renal fusion anomaly caused by disturbances in embryonic development when the kidneys are fused together in the lower or upper pole forming an isthmus. The most common disorders in urinary tract related to horseshoe kidney are ureteral pelvic junction obstruction, urinary tract infection and urolithiasis. In our study, we present a rare case of an abscess in the isthmus of horseshoe kidney after extracorporeal shockwave lithotripsy on the right kidney was performed. The patient has had recurrent urolithiasis and underwent 4 treatments on the left kidney in the past without complications.
A sight into the pathogenesis and treatment of thyroid-associated ophthalmopathy
(Publishing Office of the University of Rzeszow, 2024-06) Krzemienowska-Cebulla, Aleksandra; Puziewicz-Krzemienowska, Małgorzata; Dorochowicz, Mateusz; Matus, Iwona
Introduction and aim. Thyroid-associated ophthalmopathy (TAO), often referred to as thyroid eye disease or Graves’ ophthalmopathy, is a syndrome characterized by autoimmune inflammation affecting the eye muscles, connective tissue, and orbital fat. The aim of this literature review is to present TAO and integrate the available data in the literature regarding the pathogenesis and treatment methods. Based on these, the authors aim to examine whether, despite the extensive knowledge already available on TAO, there are still issues to be investigated. Material and methods. In this literature review, books and scientific publications in both Polish and English languages have been assessed. The search criteria included keywords such as TAO, Graves’ disease, thyroid-associated ophthalmopathy. The evaluation covered the following databases: PubMed, Scopus, Google Scholar. Analysis of the literature. Typically, the eyeball is not involved, but in exceptional cases, corneal ulceration may occur, or inflammation of the optic nerve may ensue. TAO most commonly occurs in the course of hyperthyroidism in Graves’ disease, involving up to 25–50% of cases. The coexistence of autoantigens shared between the thyroid and orbital tissues is considered the primary cause of TAO when it occurs concurrently with hyperthyroidism, later in its course, or even preceding the manifestation of hyperthyroidism, with or without concurrent thyroid dysfunction. TAO is generally bilateral, although dominance on one side is often observed. Common symptoms include eye pain, photophobia, diplopia, varying degrees of proptosis, and impaired vision. The cornerstone of treatment lies in managing hyperthyroidism, as TAO cannot be cured without it. Conclusion. First-line treatment involves glucocorticoids, with radiation therapy as a supplementary option, and in cases unresponsive to pharmacological treatment, surgical intervention may be necessary.
A study of etiological spectrum in 106 cases of pancytopenia
(Publishing Office of the University of Rzeszow, 2024-06) Singhal, Parul; Mittal, Adreena; Dixit, Ritvija
Introduction and aim. Pancytopenia is the simultaneous presence of anemia, leucopenia and thrombocytopenia. The aim of this work was to study the etiological spectrum of pancytopenia in the National capital region of India, and evaluate the relationship of pancytopenia with serum vitamin B12 levels. Material and methods. This study is of a prospective and analytical type conducted on patients attending the outpatient and inpatient department of Santosh Medical College and the Saraswathi Institute of Medical Sciences tertiary care centers in NCR. Complete blood counts and peripheral blood smear examination was used for morphological classification and typing of anemia. Bone marrow aspiration and vitamin B12 estimation was performed where required. Results. The maximum number of pancytopenia cases were etiologically attributed to megaloblastic anemia (64.2%) followed by aplastic anemia (19.8%). Malaria was attributed to 6.6% cases of pancytopenia. Iron deficiency anemia and tuberculosis both accounted for 1.9% of cases. each. A history of drug intake and mixed nutritional anemia each contributed to 2.8% of cases. Serum vitamin B12 levels showed a significant relationship with pancytopenic cases. Conclusion. In our study, the main cause of pancytopenia is megaloblastic anemia which responds very well to treatment if diagnosed correctly in time. A detailed hematological assessment along with vitamin B12 levels should be evaluated in all cases of pancytopenia irrespective of the etiological categorization.
A study of knowledge attitude practices and identification of perceived barriers towards screening for diabetic retinopathy amongst diabetics in an industrial area in western Maharashtra
(Publishing Office of the University of Rzeszow, 2023-09) Bakare, Prachi; Sethi, Sejal; Mushtaq, Iqra; Maheshgauri, Rupali; Magdum, Renu; Modani, Neha Kamalkishor; Gogate, Parikshit
Introduction and aim. Diabetic retinopathy (DR) screening is first step in prevention of diabetic retinopathy related ocular morbidity. The aim of the study was to assess the gaps in the knowledge and awareness and evaluate its effects on the attitude and practice in known diabetic patients and to assess any anticipated barriers and its association with knowledge, attitude and practices. Material and methods. A closed-ended questionnaire was given to 112 diabetics who met the inclusion criteria and visited a tertiary eye hospital in western Maharashtra. The purpose of the questionnaire was to assess knowledge, attitude and practices and perceived barriers to the study of DR. The statistical analysis was done on SPSS software. Results. In our study, 73.4% did not know about DR, suggesting poor awareness about the disease, 59.5% did not feel the need for regular eye checkup. 67 (61.1%) had no knowledge about blood sugar control level and lipid control for effective control of DR, 89 (79.5%) of participants did not know about effective treatment options. Moreover, 74 (66.1%) participants were advised for DR screening by physician, while 60 (53.5%) were willing to get screened, negative attitude was not getting screened when vision is good seen in 87 (79.1%) participants. Furthermore, 84 (75%) patient think eye checkup are expensive, 66% people believe availability of doctor is an issue. 60 (54.5%) think that eye checkups are time consuming. Conclusion. Our study demonstrated, that Spearman’s correlation coefficient between knowledge and awareness and based on attitude and practice, is 0.54, and that this is statistically significant (p<0.01). Our study has shown poor awareness and knowledge about the disease thus affecting attitude and practices, eye screening is considered expensive has been pointed out a major barrier in this study.
A study on the mycobacterial burden and phenotypic drug resistance pattern with reference to the GeneXpert Cycle Threshold values in pulmonary tuberculosis
(Publishing Office of the University of Rzeszow, 2023-12) Kashyap, Bineeta; Sarkar, Krishna; Singh, Kapil; Hyanki, Puneeta
Introduction and aim. Tuberculosis (TB) remains a significant global health challenge. Early and accurate diagnosis is crucial to prevent further transmission. The present study aimed to correlate cycle threshold values with smear microscopy and culture positivity, and determine cut-off cycle threshold values for levels of smear grade and culture positivity. Material and methods. Forty presumptive cases of pulmonary TB were included and subjected to Ziehl- Neelsen stain, culture on Lowenstein Jensen media, CBNAAT and drug susceptibility testing for first line anti-tubercular drugs. Results. Our study predicts 3+, 2+, and 1+ sputum smear grade at a cut-off of Ct value ≤ 16.74, ≤19.68, and ≤ 22.32 respectively. A strong positive correlation was found between time to culture positivity and Ct value. A cut-off of Ct value ≤22.32 predicts culture positivity with a sensitivity of 92%, and a specificity of 67%. None of the isolates showed rifampicin resistance by 1% proportion method. Conclusion. Understanding the appropriate utilization of CBNAAT Ct values and their correlation with smear microscopy grade, culture, and drug susceptibility testing can assist clinicians in early identification and prompt initiation of appropriate treatment. This knowledge can contribute to the prevention of drug resistance, reduced transmission, and a decreased disease burden associated with TB.

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