European Journal of Clinical and Experimental Medicine T.21, z. 4 (2023)

URI dla tej Kolekcjihttps://repozytorium.ur.edu.pl/handle/item/9493

Przeglądaj

Przeglądanie European Journal of Clinical and Experimental Medicine T.21, z. 4 (2023) według Temat "antioxidants"

Wpisz kilka pierwszych liter i kliknij przycisk Przeglądaj
Aktualnie wyświetlane 1 - 2 z 2
  • Obrazek miniatury
    Pozycja
    Evaluation of the antioxidant activity of Berberis jaeschkeana C. K. Schneid. fruits using the ABTS assay
    (Publishing Office of the University of Rzeszow, 2023-12) Och, Anna; Ćwikła, Agata; Orzechowska, Karolina; Cwener, Anna
    Introduction and aim. The assessment of the antioxidant activity of plant extracts is an important research direction in the search for new chemopreventive substances. The aim of this study was to demonstrate the antioxidant potential of the methanol extract of Berberis jaeschkeana C. K. Schneid fruit. Material and methods. Spectrophotometric tests were carried out: testing of the total content of polyphenols (TPC) in extracts, testing of the total content of flavonoids (TFC) in extracts. Antiradical activity was determined using the improved ABTS+• decolorization test with modifications. Results. TPC extracts was determined at the level of 43.52±2.37 mg/g, while TFC extracts was determined at the level 6.08±0.48 mg/g. The ABTS activity test showed 31.15 mg/g expressed as mg Trolox per g of dry extract. Conclusion. It was assessed that the methanolic extract of the fruit of Berberis jaeschkeana C. K. Schneid. has an antioxidant properties, what makes it a potential chemopreventive agent in civilizational diseases.
  • Obrazek miniatury
    Pozycja
    Oxidative stress and antioxidants markers in individuals with thyroid hormones dysfunction
    (Publishing Office of the University of Rzeszow, 2023-12) Omon, Emmanuel Akokhamen; Ajayi, Olawale David
    Introduction and aim. Thyroid hormone abnormalities have been associated with oxidative changes in human beings. The aim of the study was to evaluate the oxidative stress marker and antioxidants status in individuals with thyroid hormone dysfunction in Ekiti State. Material and methods. A total of eighty samples were recruited in this study comprising forty subjects with thyroid hormones dysfunction and forty apparently healthy controls. Malondialdehyde (MDA), reduced glutathione (GSH) and catalase were determined spectrophotometerically. Results. MDA was non-significantly higher (p>0.05) in subjects (4.33±0.84 nmol/mL) compared with control (4.12±0.63 nmol/mL), catalase was non-significantly higher (p>0.05) in subjects (199.36±20.21 µm/mL) compared with control (181.55±16.61 µm/mL), while GSH was significantly lower (p<0.05) in subjects (79.31±10.12 µmol/mL) compared with control (127.21±7.29 µmol/mL). Conclusion. It can be concluded that the increase in the reactive oxygen species accompanied with impairment of the antioxidant system occurs in patients with thyroid hormone dysfunction. Hypothyroidism and hyperthyroidism induces disequilibrium of the oxidative/anti-oxidative balance that can lead to subsequent development of inflammation and associated diseases.