European Journal of Clinical and Experimental Medicine T.21, z. 4 (2023)
URI dla tej Kolekcjihttps://repozytorium.ur.edu.pl/handle/item/9493
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Pozycja Genetic variants of the glucagon-like receptor-1 in obesity(Publishing Office of the University of Rzeszow, 2023-12) Nikulina, AnnaIntroduction and aim. Dysfunction of the glucagon-like peptide 1 (GLP-1)/GLP-1 receptor (GLP-1R) axis promotes obesity and metabolic disorders. The aim was to study the associations of the single nucleotide variants (SNV) GLP1R gene with proinflammatory cytokines and metabolic disorders in children with various obesity phenotypes. Material and methods. 252 children with obesity aged 6-18 years were examined. The first group (n=152) was represented by children with metabolically unhealthy obesity (MUO). The second group (n=100) consolidated of children with metabolically healthy obesity (MHO). Whole genome sequencing (CeGat, Germany) was performed in 52 children. Results. An association with the development of obesity was noted for T alleles rs61754624 (t=3.33) and rs10305457 (t=2.06); with MUO – for C alleles rs1042044 (t=2.23), rs1126476 (t=2.63), rs2235868 (t=2.82); T alleles rs61754624 (t=3.33), rs10305457 (t=2.06) GLP1R, p<0.05. In the MHO group, a correlation was found with the levels of pro-inflammatory markers IL-1β, IL-6 in the presence of the GA genotype SNV rs3765468; with hyperglycemia - GA genotype SNV rs6923761, CC genotype SNV rs1042044, AA rs6918287; hyperinsulinemia - GA genotype SNV rs3765468, GG rs10305421; triglyceridemia - AA rs6918287 of GLP1R. Conclusion. SNV rs1042044, rs3765468, rs6923761, s6918287, and rs rs10305421 GLP1R are associated with the development of MUO in individuals with MHO.Pozycja Evaluation of salivary alpha amylase activity in smokers with periodontitis, Khartoum state, 2023(Publishing Office of the University of Rzeszow, 2023) Allah, Fatima Abd Alwhab Awad; Ahmed, Hafsa Ahmed ElrheimaIntroduction and aim. Smoking widely affect oral health, including its role in the development of periodontitis. Saliva contains an antioxidant system and various enzymes. The study was designed to evaluate the activity of salivary alpha amylase among individuals who smoke and have periodontitis. Material and methods. A total of 100 participants were included, with 50 cases (cigarette smokers with periodontitis) and the remaining 50 nonsmokers with healthy periodontium as the control group. Saliva samples were collected to measure salivary alpha amylase activity. Results. Smokers with periodontitis had significantly higher levels of salivary alpha amylase compared to the control group (177.96±14.5 vs 94.04±19.6 IU/mL, p<0.001). Additionally, there was a weak negative correlation between the level of alpha amylase and the age of the patients (p=0.01, r=0.376). However, no correlation was found between the level of alpha amylase and the duration of smoking (p=0.584, r=0.079). Conclusion. There is a significant increase in salivary alpha amylase levels among smokers with periodontitis. No correlation was found between age and salivary alpha amylase levels. However, a weak positive correlation was observed between the duration of smoking and salivary alpha amylase activity.Pozycja Haemocytometric profile and plasma levels of selected cytokines in patients at various stages of cervical cancer(Publishing Office of the University of Rzeszow, 2023-12) Jimoh, Mutiu Alani; Onifade, Abdulfatah Adekunle; Abbiyesuku, Fayeofori Mpakaboari; Edem, Victory Fabian; Rahamon, Sheu Kadiri; Arinola, Olatunbosun GaniyuIntroduction and aim. Reports have shown that there is alteration in haematological and inflammatory processes in patients with cervical cancer. However, there is the dearth of information on the pattern of alteration in Nigerian patients with cervical cancer at various stages of the disease. Therefore, haemocytometric profile and plasma levels of interleukin-6 (IL-6) and IL-12 were determined in Nigerian patients with cervical cancer at various stages of the disease. Material and methods. Eighty-nine adults consisting of 49 patients with cervical cancer and 40 apparently healthy controls were enrolled into this study. Haemocytometric profile was determined using automated haematology analyzer while the plasma levels of interleukin-6 (IL-6) and IL-12 were determined using ELISA. Results. Of the participants with cervical cancer, 6.12%, 24.49%, 53.06% and 16.33% were in stages I, II, III and IV respectively. The mean plasma IL-6 level was significantly higher in patients at stage IV of the cancer compared with those in stages I, II and III. No significant differences were observed in the mean plasma IL-12 level, and the haemocytometric profile when patients in different stages of the cancer were compared with one another. Plasma IL-6 had significant positive correlation with the lymphocytes count and cancer stage but had significant negative correlation with packed cell volume (PCV), haemoglobin and total white blood cells count (WBC) in patients with cervical cancer. Conclusion. Interleukin-6 appears to play an important role in the progression of cervical cancer and could be involved in cervical cancer-associated alteration in haemocytometric profile.Pozycja Plasma level of beta endorphin in seborrheic dermatitis patients(Publishing Office of the University of Rzeszow, 2023-12) Vysochanska, Vlasta; Koval, GalinaIntroduction and aim. Defects in the epidermal barrier, changes in sebum secretion and its composition, Malassezia spp. overgrowth, endocrine, immune, and neurological disorders are the main pathogenesis items of seborrheic dermatitis (SD). “The opioid system of the skin” was considered a new target in the diagnosis and treatment of SD. The study aimed to determine beta-endorphin (BE) levels in adult patients with seborrheic dermatitis and correlate them with the severity of symptoms and itching. Material and methods. 26 healthy and 62 SD people were examined. SEDASI scale were used to estimate the severity of symptoms and intensity of itching. The determination of the beta-endorphin level was carried out by the ELISA method with the test system Human BE NBP2 (78774 Novus Biologicals). Results. BE in the SD group was higher compared to the control group (35.5 pg/mL, 22. pg/mL, p<0.001). The level of BE in seborrheic patients did not depend on age and sex but was rising with severity of symptoms. Positive correlations were found between the level of BE and the SEDASI was 0.42 (p<.001), between the level of BE and itching was 0.332 (p=0.009). Conclusion. SD patients have an increased level of BE that positively correlates with itching and disease severity.Pozycja Predictors of adverse perinatal outcomes in women at 40 weeks or more of pregnancy(Publishing Office of the University of Rzeszow, 2023-12) Sert, Zekiye Soykan; Dilmaç, Ayşegül Evren; Alkan, Ender; Sert, Ekrem TahaIntroduction and aim. To evaluate the clinical features of women at ≥40 weeks of pregnancy and the utility of obstetric Doppler indices in predicting adverse perinatal outcomes in these pregnancies. Material and methods. This prospective study was conducted at a single academic medical center between 2020 and 2022. Women aged 18 years and older with no risk factors who were at ≥40 weeks of pregnancy and delivered their babies in our hospital were included in the study. The fetal biometry, placental maturity grading, and doppler velocytometry indices of the pregnant women were evaluated. The cases were divided into two groups according to the development of adverse perinatal outcomes. The relationship between clinical features and adverse perinatal outcomes was evaluated. Results. Adverse perinatal outcomes developed in 19.6% (42) of the 214 cases. The multiple logistic regression analysis was performed to identify factors affecting perinatal outcomes. Accordingly, a maternal age of ≥35 years (odds ratio [OR]: 1.74, 95% confidence interval [CI]: 1.29–3.96, p=0.038), nulliparity (OR: 1.42, 95% CI: 1.13–4.63, p=0.040), and grade 3 placental calcification (OR: 1.98, 95% CI: 1.11–4.53, p=0.029) were independent predictors of adverse perinatal outcomes. Conclusion. Care should be taken in terms of adverse perinatal outcomes in the presence of nulliparity, a maternal age of ≥35 years, and grade 3 placental calcification in ≥40 week pregnancies.Pozycja Challenges of the COVID-19 pandemic for children and their families during home quarantine in Turkey(Publishing Office of the University of Rzeszow, 2023-12) Aydın, Bahise; Çalbayram, Nazan Çakırer; Altundağ, SebahatIntroduction and aim. The COVID-19 pandemic has caused significant changes in human life. As a result of these changes, it is important to determine the effects on the child and family life. This study was conducted to determine the challenges experienced by children and their families while home quarantine in the pandemic. Material and methods. The study was conducted with parents who had children aged 6-18, use social media, and willing to participate in the study. A total of 450 parents participated in the study. Online survey was used. Results. It was determined that some of parental daily activities decreased (doing sports, communicating with friends, engaging in hobbies) and some of them increased (personal hygiene, internet, and playing games with their children). The decreased daily activities of children (doing sports and communicating with friends) and increased activities (eating, personal hygiene, internet, and social media use, playing with toys and technological devices) were detected. There was an increase in some of the family activities (watching movies, playing games, and studying). Conclusion. It was determined that the home quarantine in the pandemic led to some negative changes in the daily living habits, lifestyles, and feelings of the family.Pozycja The effect of a physical therapy programme on the condition of upper limb muscles in patients with rheumatoid arthritis(Publishing Office of the University of Rzeszow, 2023-12) Grygus, Igor; Nogas, AnzhelaIntroduction and aim. Rheumatoid arthritis is a chronic progressive systemic disease of the connective tissue affecting the joints, mainly small, of the erosive-destructive polyarthritis type, and frequent systemic inflammatory damage to internal organs. The purpose of the research is to evaluate the effectiveness of physical therapy on the muscles of upper limbs in patients with rheumatoid arthritis by the dynamics of muscle strength and strength index. Material and methods. To determine the strength of the muscles of the affected upper limbs were used dynamometry, calculated strength index as a percentage to assess the functional ability of the affected limb. All patients were divided into control (n=92) and main (n=96) groups, taking into account the functional insufficiency of the joints. Results. After 6 months of physical therapy, muscle strength indicators in the main group of patients with 1st degree of functional joints insufficiency (FJI) in the affected right limb increased by 3.1 kg, in the left by 3 kg; with 2nd degree FJI – in the right limb by 4.2 kg, in the left – by 3.7 kg, significantly exceeded the same indicators in patients of the control group (р<0.05). Similarly, there was an increase in the strength index in the patients of the main group. Conclusion. Physical therapy technology has been developed and implemented effectively influenced the increase in muscle strength and strength index of affected upper limbs of the main group of patients with rheumatoid arthritis.Pozycja A study on the mycobacterial burden and phenotypic drug resistance pattern with reference to the GeneXpert Cycle Threshold values in pulmonary tuberculosis(Publishing Office of the University of Rzeszow, 2023-12) Kashyap, Bineeta; Sarkar, Krishna; Singh, Kapil; Hyanki, PuneetaIntroduction and aim. Tuberculosis (TB) remains a significant global health challenge. Early and accurate diagnosis is crucial to prevent further transmission. The present study aimed to correlate cycle threshold values with smear microscopy and culture positivity, and determine cut-off cycle threshold values for levels of smear grade and culture positivity. Material and methods. Forty presumptive cases of pulmonary TB were included and subjected to Ziehl- Neelsen stain, culture on Lowenstein Jensen media, CBNAAT and drug susceptibility testing for first line anti-tubercular drugs. Results. Our study predicts 3+, 2+, and 1+ sputum smear grade at a cut-off of Ct value ≤ 16.74, ≤19.68, and ≤ 22.32 respectively. A strong positive correlation was found between time to culture positivity and Ct value. A cut-off of Ct value ≤22.32 predicts culture positivity with a sensitivity of 92%, and a specificity of 67%. None of the isolates showed rifampicin resistance by 1% proportion method. Conclusion. Understanding the appropriate utilization of CBNAAT Ct values and their correlation with smear microscopy grade, culture, and drug susceptibility testing can assist clinicians in early identification and prompt initiation of appropriate treatment. This knowledge can contribute to the prevention of drug resistance, reduced transmission, and a decreased disease burden associated with TB.Pozycja Salivary microbial diversity – an investigation on possible biomarkers for polycystic ovarian syndrome from eastern India(Publishing Office of the University of Rzeszow, 2023-12) Ghosh, Kusum; Chakraborty, Shreyoshi; Chatterjee, Diptendu; Bandyopadhyay, Arup RatanIntroduction and aim. Polycystic ovarian syndrome (PCOS) is one of the major reproductive health issues, thought to be multifactorial, needs serious attention as a dual burden (health and economic) mainly for developing countries like India, due to its rapid rise (30%) in the last couple of years. Therefore, widespread and liberal screening for this disorder towards prognosis, diagnosis and intervention seems to be an urgent area of research. In this background, the present study attempts to unravel the association of salivary microbial diversity and PCOS. Material and methods. To achieve the purpose 100 clinically diagnosed PCOS individuals and 110 age matched non-PCOS participants from Bengalee Hindu caste population, West Bengal, India was considered. Obtained salivary samples were identified with 16S rDNA amplification and microbial diversity were determined by Alu I restriction enzyme digestion. Results. The present study revealed an explicit pattern of DNA fragment lengths varied between 200 bp and 225 bp in PCOs in comparison to the non-PCOS group. Conclusion. The cardinal feature of the present study as the first attempt from India envisaged, utilization of salivary microbial diversity as an additional potential and economizing biomarker for PCOS that stimulate new horizon of research in 21st century’s anthropology – the anthropology of microbes.Pozycja An in vitro study for the evaluation of morphological and biochemical characteristics of absorbable sutures coated with genistein and nicotine(Publishing Office of the University of Rzeszow, 2023-12) Chopra, Aditi; Cecil, Anju; Eshwaramoorthy, Rajalaksmanan; John, Rubin S.Introduction and aim. Inflammation, cell proliferation, matrix deposition, and tissue remodeling are all elements of the well-structured and well-coordinated process of wound repair. The aim of this study was to analyze the effect of genistein and nicotine on polyglycolic acid (PGA) and vicryl sutures. Material and methods. Genistein and nicotine were isolated and solution was prepared and the suture material PGA and vicryl were immersed in the solution and dried. They were tested for their tensile strength and degradation values after immersion in artificial saliva (on the first day and on the 14th day). The sutures were also seen under a scanning electron microscope (SEM) for its uniform coating and the mixture formulation of genistein and nicotine were tested for their anti-inflammatory and antioxidant activity using protein denaturation assay and 2,2-diphenyl-1-picrylhydrazyl assay respectively. Results. Nicotine has a high anti-inflammatory activity on the suture material, whereas Genistein has an insignificant anti-inflammatory effect. The mixture formulation has a relatively similar anti-inflammatory effect when compared to the control. The SEM analysis shows a uniform coating of the formulation on the PGA and vicryl sutures. In comparison, PGA has shown lesser tensile strength and hence higher degradation ability. Conclusion. Nicotine and Genistein affect the tensile strength and degradation properties of the sutures.Pozycja Serum levels of IFN-γ and IL-4 in hospitalised COVID-19 patients – evidence of reduced cytokine storm in discharged patients(Publishing Office of the University of Rzeszow, 2023-12) Arinola, Ganiyu Olatunbosun; Onifade, Abdulfattah Adekunle; Adigun, Kehinde; Oshingbesan, Mosunmade BabatundeIntroduction and aim. Coronavirus disease 2019 is characterised by cytokine storm and it was managed with repurposed drugs, however the effect of this treatment on cytokine storm is unknown. The aim of the study was to investigate the effect of repurposed management on serum Th1 pro-inflammation cytokine (IFN-γ) and Th2 anti-inflammation cytokine (IL-4) in COVID-19 patients. Material and methods. The levels of IFN-γ and IL-4 were determined in sera from 45 COVID-19 patients at admission followed-up till discharge after repurposed treatment using ELISA. The mean levels and proportions above normal reference ranges of IFN-γ and IL-4 were compared in COVID-19 at admission and discharge. Results. The mean values of IFN-γ and IL-4 were significantly higher in COVID-19 patients at admission compared with discharged COVID-19 patients whereas IFN-γ:IL-4 ratio was significantly higher in discharged COVID-19 patients compared with admitted COVID-19 patients. Significantly higher proportion of COVID-19 patients at discharge had IFN-γ within the normal reference ranges compared with COVID-19 patients at admission whereas the proportions of COVID-19 patients at discharge and COVID-19 patients at admission having IL-4 within the normal reference ranges were the same. Conclusion. Cytokine storm was evidenced in COVID-19 patients at admission and repurposed treatment suppressed pro-inflammation cytokine (IFN-γ) in most discharged COVID-19 patients.Pozycja Satisfaction levels of patients attending the outpatient department of a tertiary care center of India(Publishing Office of the University of Rzeszow, 2023-12) Kumar, Vijay; Biswas, Ratnadeep; Ojha, Vishnu S.; Kaushal, KishoreIntroduction and aim. Any hospital’s outpatient department (OPD) is regarded as the storefront of the facility, and patient satisfaction is an important measure of healthcare quality. Hence, this cross-sectional study was conducted in different OPDs at a tertiary care center. Material and methods. Two hundred patients were recruited, and structured personal interviews were conducted with questions based on the Patient Satisfaction Questionnaire Short Form (PSQ-18). T-tests and analysis of variance (ANOVA) were used to compare satisfaction scores between variables. Results. Upon analysis, 86% of the patients rated their overall experience as either ‘very good’ or ‘good’. Interpersonal manners (mean score±SD; 4.60±0.55), communication (4.39±0.66), general satisfaction (4.03±0.79), and technical qualities (3.86±0.57) were the domains in which the patients were most satisfied, while time spent with the doctor (3.77±0.89), accessibility and convenience (3.77±0.67), and financial aspects (3.37±0.83) were the areas that lagged. Conclusion. Satisfaction scores were found to vary significantly with gender, age, waiting times, and the number of visits per day. Regular patient satisfaction surveys should be conducted in all hospitals for devising interventions to provide patients with the best possible care.Pozycja The use of the “Talk To Me” application in the therapy of speech development delays(Publishing Office of the University of Rzeszow, 2023-12) Warmbier, Wojciech A.; Podgórska-Bednarz, Justyna; Gagat-Matuła, Anna; Perenc, LidiaIntroduction and aim. Computer techniques are increasingly used in speech therapy. The aim of the work is to present the results of the preliminary evaluation of the new “Talk To Me” application and its functionality in the treatment of speech development delays. Material and methods. The study was conducted in 3 groups of children: the study group with the use of the “Talk To Me” application, the conventional therapy group and the control group - in the case of both groups with the intervention, additional reinforcements were used. All children included in the project showed delays in speech development. The recorded age of children in all analyzed groups ranged from 2 to 6 years. In order to verify language progress, the Scale of Language Skills Acquisition in the Field of Communication Competence was used. Results. Acquisition of language skills varies depending on the group affiliation. The analysis of simple main effects for the time of measurement showed that in each group the differences between successive measurements turned out to be significant. In the study group, the increase in language skills differs from the other two groups (p<0.001). However, there is no difference between the groups with conventional and control therapy (p=1.00). Conclusion. Analysis of the research results allows us to conclude that the “Talk To Me” application is a tool that significantly affects the speed of therapy progress in the case of speech development delays concerning communicative competence.Pozycja Oxidative stress and antioxidants markers in individuals with thyroid hormones dysfunction(Publishing Office of the University of Rzeszow, 2023-12) Omon, Emmanuel Akokhamen; Ajayi, Olawale DavidIntroduction and aim. Thyroid hormone abnormalities have been associated with oxidative changes in human beings. The aim of the study was to evaluate the oxidative stress marker and antioxidants status in individuals with thyroid hormone dysfunction in Ekiti State. Material and methods. A total of eighty samples were recruited in this study comprising forty subjects with thyroid hormones dysfunction and forty apparently healthy controls. Malondialdehyde (MDA), reduced glutathione (GSH) and catalase were determined spectrophotometerically. Results. MDA was non-significantly higher (p>0.05) in subjects (4.33±0.84 nmol/mL) compared with control (4.12±0.63 nmol/mL), catalase was non-significantly higher (p>0.05) in subjects (199.36±20.21 µm/mL) compared with control (181.55±16.61 µm/mL), while GSH was significantly lower (p<0.05) in subjects (79.31±10.12 µmol/mL) compared with control (127.21±7.29 µmol/mL). Conclusion. It can be concluded that the increase in the reactive oxygen species accompanied with impairment of the antioxidant system occurs in patients with thyroid hormone dysfunction. Hypothyroidism and hyperthyroidism induces disequilibrium of the oxidative/anti-oxidative balance that can lead to subsequent development of inflammation and associated diseases.Pozycja Manifestations of post-traumatic stress in military personnel after participating in hostilities in the Russian-Ukrainian war(Publishing Office of the University of Rzeszow, 2023-12) Prykhodko, Ihor; Matsehora, Yanina; Kryvokon, Nataliya; Hunbin, Konstantin; Kovalchuk, Oleksandr; Antusheva, Nataliia; Filonenko, LesiaIntroduction and aim. Since the beginning of the Russian-Ukrainian war, many Ukrainian military personnel began to show post-traumatic stress (PTS). The purpose of the article is to identify the features of PTS exhibited in different age groups in trauma-exposed military personnel during their participation in the psychological recovery program (“Invincibility Program”). Material and methods. Ukrainian Defense Forces military personnel (n=546 males, between 20 and 60 years of age) participated in this study. All participants were divided into three age groups. Determination of the features of PTS in military personnel was carried out using psychodiagnostic tests. Results. In each age group, more than half of the participants in the “Invincibility Program” had PTS characteristics such as the sub-threshold or clinical manifestations of PTSD, adjustment disorders, low resilience to combat mental trauma, and various sleep disorders combined with somatic complaints. The data showed higher features of PTS in the younger participants and they decreased in both the 2 older sets of participants. Conclusion. The age-related features of the manifestation of PTS in military personnel must be taken into account when developing psychological recovery programs: for younger participants, such events should be carried out longer and more intensively.Pozycja Prevalence, prescription patterns, and quality of life of anaemia in adults with chronic renal disease(Publishing Office of the University of Rzeszow, 2023-12) Sandeep, Akkam; Elumalai, Karthikeyan; Williams, Helen; Salkapuram, Sunilkumar; Anandakumar, Shanmugam; Srinivasan, SivaneswariIntroduction and aim. Anaemia is a chronic kidney disease (CKD) condition characterised by a decline in glomerular filtration rate (GFR) and impaired kidney function. The aim of this study was to assess the prevalence, prescribing patterns, and quality of life of anaemia in patients with chronic renal disease who were adults (>18 years of age) at a tertiary care hospital. Material and methods. Data on demographic characteristics, laboratory results, medication prescriptions, and quality of life assessments were collected. Statistical tests were performed to determine associations between anaemia prevalence and factors like age, gender, and CKD stage. The study included 132 patients, with a gender distribution of 89 men and 43 women. Results. The most frequently prescribed drugs are epoetin (15.06%), multivitamins (14.82%), iron (10.65%), folic acid (10.22%), calcium carbonate (8.17%), calcitriol (5.6%), and omeprazole (4.22%). The cardiovascular system, blood disorders, and blood-producing organs come after the gastrointestinal tract and metabolism in the first anatomical level of the ATC classification. Conclusion. It suggests hospital audits and recommendations for improved prescription practices. Further investigation into anaemia causes and drug class appropriateness is needed, and implementing improvements could potentially improve health outcomes.Pozycja Fall risk and frailty level in older adults admitted to the emergency department with a complaint of falling(Publishing Office of the University of Rzeszow, 2023-12) Çelik, Sevim; Uğur, Neşe; Karahan, Elif; Dolu, İlknurIntroduction and aim. Falls are the second leading cause of unintentional death in the world. The study was conducted to examine the risk of falls and levels of frailty in older adult patients admitted to the emergency department due to fractures, as well as to identify the factors that influence fall risk and frailty levels. Material and methods. This cross-sectional and correlational study conducted with 155 older patients. Data collected by the patient information form, Itaki Fall Risk Scale and Edmonton Frail Scale. Results. Patients diagnosed with fracture in the emergency department had a high risk of falling with a mean score of 9.55±3.84.70.3% of the patients were frail. The one-third (30.3%) had severe frailty. There was a moderate positive correlation between the risk of falling and the mean frailty score of the older adult patients (p<0.001). Conclusion. The study showed that older adults admitted to the emergency department due to falls are at high risk of falling and the majority of them are frail. Early determination of fall risk and frailty levels in the older adults with a history of falling, prevention of falls and fractures due to falls will be beneficial in increasing the quality of life of the older adults.Pozycja The investigation of the impact of education on sexual health/reproductive health knowledge levels of nurses and midwives in extraordinary situations – evidence from Turkey(Publishing Office of the University of Rzeszow, 2023-12) Cirban Ekrem, Ebru; Kuş, Tuğçe; Erkmen, Emir; Dursun, NisanurIntroduction and aim. In extraordinary situations, sexual/reproductive health services are very important for the health of the whole society, especially women and children. The aim of this study is to assess the impact of training provided on sexual health/reproductive health during extraordinary situations on the knowledge levels of nurses and midwives. Material and methods. The research was conducted using a single-group pre-test-post-test follow-up test measurement quasi-experimental design. It was completed between November 2021–June 2022 with 140 participants working in a province in Turkey. The data were collected with the “Descriptive Information Form” and the “Sexual Health and Reproductive Health Knowledge Assessment Form for Extraordinary Situations”. Training was given with the Sexual Health/Reproductive Health Training Booklet for Extraordinary Situations. Results. It was found that the participants scored 20.82±4.47 on the Sexual Health and Reproductive Health Knowledge Assessment Form for Extraordinary Situations in the pre-test, 27.63±2.67 in the post-test, and 27.07±3.46 in the follow-up test. As a result of the training, it was determined that the difference between the scores they got from the Sexual Health and Reproductive Health Knowledge Assessment Form for Extraordinary Situations was due to the pre-test (p<0.05). Conclusion. In the study, the participants scored above the average in the pre-test, while they achieved significantly higher scores in the post-test and follow-up test. This shows that the training given to nurses and midwives is effective.Pozycja Determination of attitudes and practices of nurses working in the psychiatry clinic towards physical health care of individuals with severe mental illness(Publishing Office of the University of Rzeszow, 2023-12) Kurtoğlu, Hande Yardımcı; Çelik İnce, SevecenIntroduction and aim. Individuals with severe mental illness experience more physical health problems than the general population and their life expectancy may be shorter. Therefore, the physical care provided to these patients is very important.This study aims to examine the attitudes, practices, and training needs of nurses working in psychiatry clinics towards physical health care of individuals with severe mental illness. Material and methods. This descriptive, cross-sectional study collected data from 139 nurses in psychiatry clinics using the Personal Information Form and Physical Health Attitude Scale. Results. This study found that nurses often provide physical healthcare as part of their routine care for individuals with severe mental illness. However, the study also revealed that nurses require additional education to better provide this type of care. Despite this, the nurses generally displayed a positive attitude towards physical health. The average total score on the physical health attitude scale was 80.33±10.14. Conclusion. The study concluded that nurses have a positive attitude towards physical health in general. The role of nurses working in psychiatry clinics is crucial in evaluating and caring for physical health of individuals with severe mental illness, as it is an important aspect of holistic nursing care. It is necessary to make nurses aware of their shortcomings in providing physical health care and support them in improving in this area.Pozycja Nursing students’ awareness of health-promoting lifestyle profile and sustainable development goals – a quasi-experimental study(Publishing Office of the University of Rzeszow, 2023-12) Dolu, lknur; Bozkurt, Feyza DemirIntroduction and aim. Health promotion is one of the common elements of sustainable development goals. The aim of this study was to identify the impact of “Healthy Life and Environment” course on nursing student’s awareness of sustainable development and healthy life, and health-promoting lifestyle behaviours. Material and methods. A pre-, and post-test control group quasi-experimental research design was used. One nursing department located in Nortwest Blacksea region, Turkey. A total of 160 nursing students pursuing bachelor nursing programs. Students from first and second years who enrolled in the “Healthy Life and Environment” course assigned to intervention group, those who did not attend the course assigned to control group. Results. Female students had higher level of HPLP II and SDA than male. SDA had a weak correlation with HLA and a very week correlation with HPLP II at baseline. Intervention group had an improvement of .03 points in HPLP II, no change in HLA, and a slight improvement of .01 points in SDA, however these differences were not statistically important. Conclusion. The results of our study highlighted that nursing curricula need to be modified to incorporate sustainable development methodology using student-centered learning.