A study on the mycobacterial burden and phenotypic drug resistance pattern with reference to the GeneXpert Cycle Threshold values in pulmonary tuberculosis

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dc.contributor.authorKashyap, Bineeta
dc.contributor.authorSarkar, Krishna
dc.contributor.authorSingh, Kapil
dc.contributor.authorHyanki, Puneeta
dc.date.accessioned2023-12-29T13:29:52Z
dc.date.available2023-12-29T13:29:52Z
dc.date.issued2023-12
dc.descriptionThe study was approved by the Institutional Review Board of the Institutional Ethics Committee-Human Research of the University College of Medical Sciences (IEC-HR/ 2019/41/68).
dc.description.abstractIntroduction and aim. Tuberculosis (TB) remains a significant global health challenge. Early and accurate diagnosis is crucial to prevent further transmission. The present study aimed to correlate cycle threshold values with smear microscopy and culture positivity, and determine cut-off cycle threshold values for levels of smear grade and culture positivity. Material and methods. Forty presumptive cases of pulmonary TB were included and subjected to Ziehl- Neelsen stain, culture on Lowenstein Jensen media, CBNAAT and drug susceptibility testing for first line anti-tubercular drugs. Results. Our study predicts 3+, 2+, and 1+ sputum smear grade at a cut-off of Ct value ≤ 16.74, ≤19.68, and ≤ 22.32 respectively. A strong positive correlation was found between time to culture positivity and Ct value. A cut-off of Ct value ≤22.32 predicts culture positivity with a sensitivity of 92%, and a specificity of 67%. None of the isolates showed rifampicin resistance by 1% proportion method. Conclusion. Understanding the appropriate utilization of CBNAAT Ct values and their correlation with smear microscopy grade, culture, and drug susceptibility testing can assist clinicians in early identification and prompt initiation of appropriate treatment. This knowledge can contribute to the prevention of drug resistance, reduced transmission, and a decreased disease burden associated with TB.eng
dc.identifier.citationEuropean Journal of Clinical and Experimental Medicine T. 21, z. 4 (2023), s. 730–735
dc.identifier.doi10.15584/ejcem.2023.4.11
dc.identifier.eissn2544-1361
dc.identifier.urihttps://repozytorium.ur.edu.pl/handle/item/9501
dc.language.isoeng
dc.publisherPublishing Office of the University of Rzeszow
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Poland*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/*
dc.subjectCBNAAT
dc.subjectcycle threshold values
dc.subjectrifampicin resistance
dc.subjectsmear microscopy
dc.subjecttuberculosis
dc.titleA study on the mycobacterial burden and phenotypic drug resistance pattern with reference to the GeneXpert Cycle Threshold values in pulmonary tuberculosis
dc.typearticle
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European Journal of Clinical and Experimental Medicine T.21, z. 4 (2023)