Identification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction

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dc.contributor.authorManivannan, Kavitha
dc.contributor.authorRamasamy, Malathi
dc.contributor.authorSundaresan, Uma
dc.contributor.authorMoustafa, Samar M.
dc.contributor.authorSherloumay
dc.contributor.authorMariyam, Safna
dc.date.accessioned2024-03-28T19:20:56Z
dc.date.available2024-03-28T19:20:56Z
dc.date.issued2024-03
dc.descriptionExperiment has been approved by administration of Tharb camel Hospital.
dc.description.abstractIntroduction and aim. Brucellosis is a zoonotic disease. Experimental clinical and laboratory diagnosis is still facing problems in identifying the organism. The present study will diagnose a Brucella infection in camel blood in Qatar using serological assays. Isolation and identification were performed on a camel blood sample. Brucella in bacterial isolates was determined by real-time polymerase chain reaction (RT-PCR) as a gold standard test. Material and methods. A total of 220 samples, 200 random serum samples, and 20 EDTA blood samples were selected among the above-mentioned random samples, and 20 serum samples from camel handlers were collected from Al Shahaniya prov ince, Qatar. The Rose Bengal test (RBT), buffered antigen plate agglutination test (BAPAT), and enzyme linked immunosorbent assay (cELISA) for the monoclonal antibody in serum samples were performed using commercially available kits. For the molecular detection of Brucella, conventional PCR and real-time PCR (GPS kit) were used for the genus-specific insertion sequence IS711. Brucella melitensis (MICROBOSS Hightech GmbH kit) was used to identify subspecies. Results. The results identified by vitek2 compact (30%) showed B. melitensis in 6 samples out of 20 isolates. Both conventional (66.67%) and RT-PCR (83.33%) analyses supported this, demonstrating the presence of Brucella. These tests also showed that Brucella species were present in Rose Bengal 182/200 (91%), BAPAT 182/200 (91%), and cELISA (90%) 180/200 in camel serum. Conclusion. To conclude, the prevalence of brucellosis in dromedary camels is higher in this region, and as a matter of urgency, measures should be taken to control the disease.eng
dc.identifier.citationEuropean Journal of Clinical and Experimental Medicine T. 22, z. 1 (2024), s. 94–101
dc.identifier.doi10.15584/ejcem.2024.1.17
dc.identifier.eissn2544-1361
dc.identifier.urihttps://repozytorium.ur.edu.pl/handle/item/10362
dc.language.isoeng
dc.publisherPublishing Office of the University of Rzeszow
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 Poland*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/pl/*
dc.subjectBrucella melitensis
dc.subjectbrucellosis
dc.subjectcamels
dc.subjectconventional PCR
dc.subjectRT-PCR
dc.subjectserological assays
dc.titleIdentification of Brucella melitensis from camel’s blood by vitek2 and real time polymerase chain reaction
dc.typearticle
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European Journal of Clinical and Experimental Medicine T.22, z. 1 (2024)