Introduction and aim. The central nervous systems control salivary pH and Flow; hence it is considered as stress biomarker. To correlate the relationships linking test performance and the cognitive along with affective aspects for the stress of the exams, flow rate and pH levels of saliva. Material and methods. Cross-sectional research was carried out on the day of their final exam and after three months. 90 dental college students provided saliva samples for measuring pH and salivary flow rate. The saliva was collected to measure stress, anxiety, and wellbeing. Results. Salivary flow and pH were increased in the after-exam period. This is mostly due to less threat, stress, and anxiety perception. Conclusion. According to this study, perceived stress can affect salivary flow rate and pH levels, which can be used to gauge the degree of physiological reactions accurately, conveniently, and affordably to tests and variable realistic stresses.