Evaluation of neutrophil phagocytic, complement functions, and cytokines expression among diabetic patients in Abuja, Nigeria

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dc.contributor.authorBabandina, Musa Muhammad
dc.contributor.authorMainasara, Abdullahi Suleiman
dc.contributor.authorBakare, Mustapha
dc.contributor.authorEmeribe, Anthony Uchenna
dc.contributor.authorShuwa, Halima Ali
dc.contributor.authorHaruna, Shamsuddeen
dc.contributor.authorMuhammad, Aminu Said
dc.contributor.authorAbdullahi, Idris Nasir
dc.date.accessioned2019-11-04T07:59:19Z
dc.date.available2019-11-04T07:59:19Z
dc.date.issued2019
dc.descriptionAuthors deeply appreciate staff of immunology/ laboratory of UATH for logistic and technical assistance proof reading this manuscript before submission.pl_PL.UTF-8
dc.description.abstractIntroduction. Inflammatory response in Diabetes Mellitus (DM) begins with chronic sub-clinical inflammations as a result of insulin resistance and activation of both innate and adaptive immune system as the disease progresses to complicated diabetes. Hence, the present study investigated the neutrophil phagocytic, complement function (CH50), and some cytokine profiles among diabetic and non-diabetic patients attending the National Hospital in Abuja, Nigeria. Aim. To evaluate the neutrophil phagocytic, complement function (CH50), and some cytokine profiles among post-operative septic diabetic and post-operative septic non-diabetic patients at the National Hospital in Abuja, Nigeria. Material and methods. Subjects were recruited by convenient sampling technique through interviewer-administered questionnaires. Subsequently, blood samples were collected. Fasting blood sugar (FBS) (mmol/L) was determined using glucose oxidase method. Neutrophil function test (Fmol/phag) was assayed using nitroblue tetrazolium reduction test (NBT). Hemolytic complement function (CH 50) test was conducted using serum harvested from sheep sensitized with human group (ORh D +ve) red blood cells. While serum Interleukin-4, -6, -10 and TNF- α were determined using Enzyme Linked Immunosorbent Assay (ELISA). Results. Mean ±} Standard deviation (SD) of FBS concentration of 10.5 ±} 1.3 (mmol/|L) among diabetic and 4.7 ±} 0.9 (mmol/L) among non-diabetics was recorded. There is a decrease in neutrophil phagocytic function with a mean ±} SD of 5.4 ±} 2.1 (Fmol/ phag) in diabetics compared to 9.2 ±} 2.1 (Fmol/phag) in non-diabetics. Similarly, complement (CH 50) function and C-reactive protein were significantly lower in diabetics when compared to non-diabetics (p<0.001). There was a significant difference in IL-6 concentration between diabetics and non-diabetics groups, but no significant difference was observed in TNF-α, IL-4 and IL-10 concentrations between study groups (p>0.05). TNF-α and IL-6 was significantly higher in diabetics with cardiovascular disorders compared to non-diabetics subjects with cardiovascular disorders (p<0.001). Conclusion. Findings from this study revealed the association of complement, neutrophil phagocytic function, CRP and IL-6 among septic diabetic patients,. In addition TNF-α and IL-6 expression was higher in DM patients with cardiovascular disorders.pl_PL.UTF-8
dc.identifier.citationEuropean Journal of Clinical and Experimental Medicine T. 17, z. 3 (2019), s. 229–235pl_PL.UTF-8
dc.identifier.doi10.15584/ejcem.2019.3.5
dc.identifier.eissn2544-1361
dc.identifier.issn2544-2406
dc.identifier.urihttp://repozytorium.ur.edu.pl/handle/item/5061
dc.language.isoengpl_PL.UTF-8
dc.publisherWydawnictwo Uniwersytetu Rzeszowskiegopl_PL.UTF-8
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Międzynarodowe*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectdiabetes mellituspl_PL.UTF-8
dc.subjecteffector moleculespl_PL.UTF-8
dc.subjectpro-inflammatory markerspl_PL.UTF-8
dc.titleEvaluation of neutrophil phagocytic, complement functions, and cytokines expression among diabetic patients in Abuja, Nigeriapl_PL.UTF-8
dc.typearticlepl_PL.UTF-8
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European Journal of Clinical and Experimental Medicine T.17, z. 3 (2019)