Przeglądanie według Autor "Kawczyk-Krupka, Aleksandra"

Aktualnie wyświetlane 1 - 6 z 6

Coagulation markers in diagnostic and monitoring of thromboembolic complication in COVID-19
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Czarnecka-Czapczyńska, Magdalena; Bartusik-Aebisher, Dorota; Krupka-Olek, Magdalena; Aebisher, David; Cieślar, Grzegorz; Latos, Wojciech; Kawczyk-Krupka, Aleksandra
Introduction. Coronavirus disease 2019 (COVID-19) was first observed in China in Wuhan city, Hubei province in December, 2019, and specified as a pandemic by the World Health Organization (WHO). COVID-19 is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARSCoV2). Aim. The aim of this article is to discuss epidemiology of thromboembolic complication in COVID-19. Material and methods. This article is a review done in regards to discuss clinical features of the anticoagulation treatment in COVID-19. Analysis of the literature. A review is discussed an anticoagulation treatment in 41 manuscripts. Conclusion. Most commonly coagulation abnormalities in patient with COVID-19 is mild thrombocytopenia. Apart from their typical role in thrombosis and hemostasis, platelets mediate key aspects of immune and inflammatory.
Effect of photodynamic therapy with hypericin on the secretion of selected cytokines of colorectal cancer cells tested in vitro
(Publishing Office of the University of Rzeszow, 2023-06) Międzybrodzka, Anna; Kawczyk-Krupka, Aleksandra; Aebisher, David; Cieślar, Grzegorz; Czuba, Zenon Paweł
Introduction and aim. Photodynamic therapy is a complex process involving the introduction of photosensitizers into the patient’s body and irradiation of them in order to destroy the lesion, and activate the immune system. An important role in photodynamic therapy is played by photobiochemical and physical mechanisms that affect the tumor vessels and lead to the death of the damaged cell. The aim of the study is to determine the effect of photodynamic therapy with the use of Hypericin (Hyp) on the secretion of selected cytokines by colorectal cancer cells. Material and methods. Two colorectal cancer cell lines SW480 and SW620 were used in the study. Cells treated Hypericin were exposed to visible light. Then cell viability was determined by the MTT assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. Assays were performed for control samples without hypericin and light exposure, with Hyp without light exposure, without Hyp and irradiated with light, and test samples with Hyp and light exposure. Results. In the experiment we reveal, that Hyp- photodynamic activity does not influence the secretion of cytokines. Conclusion. The obtaining results confirming the destructive effect of Hyp- PDT on the colon cancer cells, show a possibility of extending the indication for photodynamic therapy using Hyp, qualification of precancerous changes.
Effects of hypericin-mediated photodynamic therapy on GM-CSF, MIF, VCAM-1 and ICAM-1 secretion in colorectal cancer cells in vitro
(Publishing Office of the University of Rzeszow, 2023-06) Międzybrodzka, Anna; Kawczyk-Krupka, Aleksandra; Aebisher, David; Cieślar, Grzegorz; Czuba, Zenon Paweł
Introduction and aim. Photodynamic therapy with hypericin (HYP-PDT) is gaining importance as a potential treatment method for malignant neoplasms. The following study investigated whether HYP-PDT influences the secretion of certain factors of colon cancer cells in vitro. Material and methods. Two colon cancer cell lines were used in this experiment: SW480 and SW620. The cells were properly prepared and then treated with photodynamic therapy with hypericin at concentrations of 0.25 μM and 0.5 μM and irradiation at the doses of 1 J/cm 2 , 5 J/cm 2 and 10 J/cm 2 . After using HYP-PDT, changes in the concentrations of four factors: GM-CSF, MIF, VCAM-1 and ICAM-1 were analyzed in the test tubes. Results. In the case of SW480 cells: a notable decrease in GM-CSF, MIF, VCAM-1 and ICAM-1 secretion was noted after HYP-PDT. In the case of SW620 cells, after HYP-PDT: no effect on GM-CSF secretion was noted; it inhibited the secretion of VCAM-1 and MIF and increased the secretion of ICAM-1. Conclusion. Photodynamic therapy with hypericin shows immunomodulatory potential in an in vitro cell line experiments. This may indicate its possible ability to modify the course of malignant tumors and therefore requires further research.
New endoscopic treatment methods for PPI-resistant GERD
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Sosna, Barbara; Bartusik-Aebisher, Dorota; Cieślar, Grzegorz; Kawczyk-Krupka, Aleksandra; Latos, Wojciech
Introduction. Gastroesophageal reflux disease (GERD) is a common disease with the highest prevalence in North America. Up to 40% of patients report persistent gastroesophageal reflux disease (GERD) symptoms despite proton pump inhibitor (PPI) therapy. Aim. The aim of this article is to complete discuss the GERD characterized by heartburn and/or regurgitation symptoms. Material and methods. We discuss here the evidence for medical therapy for PPI nonresponsive GERD. Analysis of the literature. GERD may present with a variety of other symptoms, including water brash, chest pain or discomfort, dysphagia, belching, epigastric pain, nausea, and bloating. In addition, patients may experience extraesophageal symptoms like cough, hoarseness, throat clearing, throat pain or burning, wheezing, and sleep disturbances. Conclusion. There has been an increase in GERD prevalence. GERD is one of the most common gastrointestinal disorders managed by gastroenterologists and primary care physicians.
New fluorescent imaging technics in gastrology
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Sosna, Barbara; Bartusik-Aebisher, Dorota; Cieślar, Grzegorz; Kawczyk-Krupka, Aleksandra; Latos, Wojciech
Introduction. There is a need to develop a new imaging technique in medicine. Gastroenterology is the branch of medicine focused on the digestive system and its disorders therefore for this branch is needed to detect all problems affecting the gastrointestinal tract. Aim. The aim of this article is to complete discuss the possibility of the new fluorescent imaging technics in gastrology to use innovative screening to identify individuals at an early stage. Material and methods. We discuss here imaging techniques such as include x-rays, computed tomography, scans, and magnetic resonance imaging in gastrology. Spectroscopy is the study of the formation and interpretation of spectra resulting from the interaction of all types of radiation on matter understood as a community of atoms and molecules. Various spectroscopic techniques are obtained by combining different types of radiation with different ways of its interaction with the test sample. They provide the opportunity to obtain detailed information about the tested substance – from its atomic composition, through its chemical structure, to its surface structure. Analysis of the literatue. The tissue fluorescence spectrum can be obtained by: (1) autofluorescence, or natural or primary fluorescence, i.e. by direct irradiation of the tissue with laser radiation (laser-induced fluorescence – LIF) and (2) photodynamic diagnosis (PDD), where spectrum analysis is preceded by systemic or local administration of the photosensitizer. Conclusion. The use of fluorescence imaging in colon cancer patient has potential to improve quality of treatment and diagnosis.
The role of new biomarkers for the diagnosis and treatment of colon cancer
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Czarnecka-Czapczyńska, Magdalena; Bartusik-Aebisher, Dorota; Krupka-Olek, Magdalena; Aebisher, David; Cieślar, Grzegorz; Latos, Wojciech; Kawczyk-Krupka, Aleksandra
Introduction. Colorectal cancer may be benign or malignant. According to the World Health Organization and CDC, it is the second most common cancer worldwide, after lung cancer. The mortality of colorectal cancer has been dropping for more than 20 years due to the improvements in screening techniques and treatments. Aim. The aim of this article is to discuss the role of new biomarkers for the diagnosis and treatment of colon cancer. Material and methods. This article is a review done in regards to discuss the role of new biomarkers for the diagnosis and treatment of colon cancer. Analysis of the literature. A review is discussed the role of new biomarkers for the diagnosis and treatment of colon cancer using current literature. Conclusion. The screening tests based on diagnostic new biomarkers may cause faster detection of cancer and risk factors, and provide prognostic information in order to adjust individual therapy.