Przeglądanie według Autor "Karaselek, Mehmet Ali"

Aktualnie wyświetlane 1 - 2 z 2

A family screening of CD19 gene mutation by PCR-RFLP
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2022) Karaselek, Mehmet Ali; Kapaklı, Hasan; Güner, Şükrü Nail; Kurar, Ercan; Küççüktürk, Serkan; Keleş, Sevgi; Reisli, İsmail
Introduction and aim. Mutation(s) in the gene encoding the CD19 molecule affect CD19 protein expression and primary immunodeficiency (PID) occurs. The PCR-RFLP method, which is faster and cheaper than other mutation detection methods, is rarely used in the diagnosis of PID. The study aimed to genetically identify CD19 deficiency, which is a PID, using the PCR-RFLP method. Material and methods. A total of 8 patients and two healthy controls were included in the study and the relevant region genotypes in the CD19 gene were determined by performing PCR-RFLP analysis. Results. The index case, newborn baby and mother were also included in the study. It was determined that the index case (P6) was homozygous mutant, the newborn baby (P7) and mother (P8) had heterozygous genotype. Based on this situation, one child (P1) was found to be homozygous mutant, mother (P2), father (P3) and other children (P4 and P5) had heterozygous genotype in the family, which was determined to be related to the first case. Conclusion. In our study, it has been shown that PCR-RFLP is a method that can be used in the diagnosis of PID by determining genotypes using PCR-RFLP, and especially in terms of rapid genetic testing of family screenings.
The effects of acute high intensity interval training on hematological parameters and neutrophils to lymphocytes ratio in elite taekwondo athletes according to gender
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2022-12) Işık, Bülent; Küççüktürk, Serkan; Yüksel, Mehmet Fatih; Boyalı, Ekrem; Karaselek, Mehmet Ali; Erdağı, Kenan
Introduction and aim. Intense taekwondo (TKD) training, it is important to know the exercise-induced hematological and inflammatory conditions and to develop conditions suitable for physiological needs. The aim of study is to investigate the effects of TKD-specific training containing a high-intensity interval training (HIIT) component hematological parameters and on systemic inflammatory biomarkers between gender. Material and methods. The research was carried out with twenty-four elite TKD athletes (12 female, 12 male). 90 minutes of TKD-specific unit training, including 50 minutes of HIIT component was applied to the athletes. Hematological parameters included erythrocytes, platelets, leukocytes and their subgroups and inflammatory biomarkers. Results. With the effect of TKD-specific HIIT, erythrocytes and hematocrit values decreased regardless of gender (p=0.003, p<0.001, respectively). Platelet values decreased in male and increased in female (p=0.637). White blood cells and neutrophil (p<0.001) and inflammatory biomarkers neutrophils-to-lymphocytes ratio (NLR) and platelet-to-lymphocytes ratio PLR (p<0.001, p=0.022, respectively) increased regardless of gender. Lymphocyte decreased marginally significantly (p=0.059). Conclusion. This study showed that TKD-specific HIIT increased systemic inflammatory conditions and decreased oxygen-carrying blood parameters. These fundamental findings can contribute to training science in arranging a specific taekwondo training program and sports medicine in protecting the health of athletes.