Przeglądanie według Autor "Bogut, Agnieszka"

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Analysis of the bacterial biofilm formation in different models of the in vitro culture
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Bogut, Agnieszka; Magryś, Agnieszka
Introduction. Microtiter plate assay (MPA) remains one of workhorses of in vitro biofilm research but it requires optimization of experimental conditions to fulfill the biofilm formation requirements of different bacterial pathogens. Aim. The aim was to determine the effect of TSB and RPMI1640 culture media and selected culture variables (O2 vs. 5% CO2, extended incubation time) on the biofilm production by bacteria commonly involved in biofilm-related infections: Enterococcus faecalis (EF), Escherichia coli (EC), Staphylococcus aureus (SA), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP). Material and methods. The investigation was performed using the MPA with crystal violet. Results. Statistically significant (p<0.05) increase in biofilm production between 24h and 72h time points was observed for EF (TSB o2, RPMIo2 and RPMIco2), EC (TSBo2), SA (TSBo2, TSBco2), KP (TSBo2, TSBco2), PA (RPMIco2, TSBco2). The TSB caused a significantly greater stimulation of biofilm production compared to RPM1640. It outcompeted RPMI1640 irrespective of the atmospheric conditions for SA and KP and under aerobic conditions for EF. Conclusion. Although the TSB provided the most optimal conditions for biofilm production, the process was influenced by the strain type, atmospheric conditions and period of cultivation which limits the ability to design a single universal model of the in vitro biofilm investigation.
The role of opportunistic Corynebacterium spp. in human infections
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Olender, Alina; Bogut, Agnieszka; Bańska, Agata
Introduction. The non-diphtherial corynebacteria (diphtheroids, “coryneform” bacteria) have been increasingly recognized as causative agents of human infections. Aim. To provide an overview of the role of non-diphtherial Corynebacterium species in human infections. Material and methods. Analysis of the literature data found in the PubMed database. Results. The role of diphtheroids - inherently low-virulent microorganisms considered members of the human microbiota – as potential pathogens has been linked to specific risk factors including immunosuppression, implantation of biomaterials and invasive medical procedures. Their pathogenic potential is primarily associated with frequent multidrug resistance, the ability to adhere to biotic and abiotic surfaces and/or to form biofilm as well as with internalization, intracellular survival and persistence within human cells. The most common infections include bacteremia, sepsis, endocarditis, meningitis, urinary tract infections, respiratory tract infections, wound and skin infections, and endophthalmitis. The leading species are C. jeikeium, C. striatum, C. urealyticum, C. amycolatum, and C. pseudodiphtheriticum. Conclusion. Opportunistic corynebacteria can be responsible for a wide range of infections which can be expected to increase in frequency in the future due to an enlarging population of patients with predisposing risk factors but also due to the increasing problem of antibiotic resistance in this group of bacteria.