Przeglądanie według Autor "Aebisher, David"

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19F MRI As a tool for imaging drug delivery to tissue and individual cells
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2017) Bober, Zuzanna; Aebisher, David; Ożóg, Łukasz; Tabarkiewicz, Jacek; Tutka, Piotr; Bartusik-Aebisher, Dorota
Over the past few decades, magnetic resonance imaging (MRI) has proven to be extremely successful in medical applications. More recently, the biomedical applications of MRI have been gaining more use in the field of clinical pharmacy. In 1977, perfluorocarbon compounds (PFC), which form emulsions that can carry drugs, were analyzed by 19F MRI and emulsified PFC compounds have been investigated as potential blood substitutes since the early 1960s and now a wide variety of PFC compounds are currently available as 19F MRI biomarkers. Molecules with 19F substituents are particularly attractive for use in drug tracking by 19F MRI due to 100% 19F abundance, high 19F MRI sensitivity (0.83 relative to 1H MRI) and an impressively large chemical shift range (400 ppm). Another benefit in the use of 19F MRI is a zero background signal in biological samples due to lack of endogenous fluorine. Therefore, drugs containing fluorine atom have potential for 19F MRI imaging drug delivery to tissue. This article will review recent developments in the use of 19F MRI in imaging drug delivery to tissue and individual cells.
A 16-year-old patient with Charcot Marie Tooth disease in variant c.217G>C of the INF2 gene and focal glomerulosclerosis – a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Przygoda, Maria; Matias, Dawid; Jurczak, Maciej; Sokołowska, Aldona; Raba, Karolina; Wołkanowski, Juliusz; Rydzanicz, Małgorzata; Kosińska, Joanna; Płoski, Rafał; Aebisher, David; Pyrkosz, Antoni
Introduction. Charcot Marie Tooth disease (CMT) is currently one of the most commonly diagnosed and commonly hereditary sensorimotor neuropathies. Concluding from the literature, this is the first study describing the case of a patient with CMT disease in the c.217G> C variant of the INF2 gene and focal segmental glomerulosclerosis. Aim. To present a case of a 16-year-old patient suffering from CMT disease in variant c.217G> C of the INF2 gene and focal glomerulosclerosis. Description of the case. The text describes the CMT disease in a patient who underwent the WES / WGS-NGS genetic test and found a mutation within the INF2 gene at the chromosomal position hg38 14: 104701582-G> C, cDNA level c.217 G> C , notation at the p protein level (Gly73Arg). Genotype record according to Human Genome Variation Society: NM_022489.4: c. [217G> C]; [217 =]. The publication includes data on genetics, molecular mechanisms of the disease, diagnostic methods, rehabilitation and surgical treatment. Conclusion. CMT disease is a heterogeneous group of diseases caused by mutations in various genes. The incidence of this pathology has increased significantly in the last century. Currently, there are no treatments available to combat this disease, and symptomatic treatment is the only treatment available.
Adrenomyeloneuropathy – a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Leksa, Natalia; Aebisher, David; Galiniak, Sabina; Uberman-Kluz, Dominika; Bartosz, Seweryn; Bartusik-Aebisher, Dorota
Introduction. Adrenoleukodystrophy is a genetic disorder linked to the X chromosome, in which the peroxisomal beta-oxidation process is disturbed. It is a metabolic disease that results in the accumulation of very long chain fatty acids (VLCFAs - very long chain fatty acids) responsible for the symptoms of damage to the adrenal cortex, gonads and the brain. Aim. A clinical case is reported. Description of case. This article describes the case of a 64-year-old woman who had neurological symptoms for many years, gradually increasing without significant improvement after the treatment (periodic steroid therapy). Conclusion. Based on tests (including the determination of the ABCD1 gene and very long chain fatty acids - VLCFA), adrenoleukodystrophy was suspected.
An iliac artery aneurysm
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2020) Leksa, Natalia; Leksa, Dawid; Gałuszka, Łukasz; Uryniak, Adam; Aebisher, David; Galiniak, Sabina; Bartusik-Aebisher, Dorota
Introduction. Isolated common iliac artery aneurysms (CIAs) are rare. The rarer are rupture of the common iliac artery. We can treat them surgically: classic or endovascular. Open surgery has a main role in the management of aneurysms not suitable for repair by endovascular techniques. Aim. The aim of the study is to present the possibility of occurrence of such a disease at a young age, to indicate non-characteristic symptoms that may predict the above-mentioned disease entity at the level of a primary care physician or SOR. Review of medical publications from 2000-2019 regarding guidelines or methods of conduct in that cases. Description of the case. A patient 39 years old, admitted to the Department of Vascular Surgery, in an interview: about 15 years ago severely beaten, as a result of injuries total loss of hearing, memory and basic skills such as: speech, writing, reading, hospitalized over 6 months. Conclusion. Good rehabilitation and patient persistence restored cognitive skills. Advances in surgical has lowered the mortality and morbidity rates even in patients.
Anticancer properties of Viburnum
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2018) Stępień, Agnieszka; Aebisher, David; Bartusik-Aebisher, Dorota
Aim. The aim of this paper is to provide an overview of the anticancer properties of different species of Viburnum. Materials and methods. Forty nine papers that discuss the medicinal history and current research of Viburnum species as phytotherapeutic agent were used for this discussion. Literature analysis. The results of scientific research conducted in vitro indicate that the compounds present in the extracts of Viburnum significantly affect the development of cancer cells such as leukemia, cervical cancer, breast, colon, lung, skin and stomach. This indicates that they may be used as a therapeutic agent to support oncological therapies.
ASL (Arterial Spin Labeling) – historical and current perfusion MR methods
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2017) Guz, Wiesław; Bober, Zuzanna; Ożóg, Łukasz; Truszkiewicz, Adrian; Przypek, Aneta; Aebisher, David; Bartusik-Aebisher, Dorota; Urbanik, Andrzej
Despite continuous scientific and technological advances in MR imaging, MR perfusion methods have not yet been widely deployed for routine clinical diagnostics. This is especially true for ASL (arterial spin labelling) methods used to evaluate cerebral perfusion. This method does not require a contrast agent, as new discoveries about gadolinium accumulation in the cerebellum and brain nucleus appear to be a valuable asset and provide the opportunity to be more widely deployed in clinical practice. The aim of this paper is to present the historical determinants of the development of MR perfusion techniques, the disadvantages and advantages and possible clinical applications and prospects of ASL development. Both historical articles published on MR in the 1990s and current research between 2006-2016 have been reviewed. The authors present in the work the MR perfusion method focusing on issues related to arterial spin labeling (ASL). Historically CASL (continuous ASL) and PCSL (pulsed ASL) techniques have been described and the pseudocontinuous ASL (pseudocontinuous ASL) 3D technique presents its technical and methodological considerations, advantages and disadvantages over previous methods. The methods of test protocol optimization and accompanying artifacts, as well as possible clinical applications and development perspectives, have been described.
Biological properties of Cistus species
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2018) Stępień, Agnieszka; Aebisher, David; Bartusik-Aebisher, Dorota
Aim. This paper presents a review of scientific studies analyzing the biological properties of different species of Cistus sp. Materials and methods. Forty papers that discuss the current research of Cistus sp. as phytotherapeutic agent were used for this discussion. Literature analysis. The results of scientific research indicate that extracts from various species of Cistus sp. exhibit antioxidant, antibacterial, antifungal, anti-inflammatory, antiviral, cytotoxic and anticancer properties. These properties give rise to the possibility of using Cistus sp. as a therapeutic agent supporting many therapies.
Bladder leiomyoma: diagnostics, laparoscopy, and treatment
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2020) Godlewski, Dominik; Pszczółkowski, Patryk; Fedus, Tadeusz; Aebisher, David
Introduction. Leiomyosarcoma is the most common benign tumor of the bladder. However, it is only a small percentage of all proliferative changes of this organ. Aim. Presentation based on two clinical cases of diagnostics and surgical treatment from laparoscopic access of bladder leiomyoma. Description of the cases. Case 1. The work contains a diagnosis based on transvaginal ultrasound (TVUS), cystoscopy and computed tomography (CT), as well as partial laparoscopic bladder re section of leiomyoma in young women. Case 2. The case of young women with the histopathological result confirmed bladder leiomyoma. Conclusions. Leiomyoma is a rare bladder cancer. Laparoscopic bladder resection is a safe method that leads to healing.
Bladder Mullerianosis – a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Ostańska, Elżbieta; Aebisher, David; Kaznowska, Ewa; Bartusik-Aebisher, Dorota
Introduction. Bladder mullerianosis is a rare and proliferative lesion that contains at least two types of ectopic Mullerian tissue in its wall. Aim. To present case of bladder mullerianosis. Description of case. The text contains a description of a clinical case of a 50-year-old woman admitted to a gynecological ward due to diarrheal symptoms and abdominal pain. In a CT examination of the abdominal cavity with contrast, within the posterior left-sided wall of the bladder a 43x25mm proliferative lesion suggestive of neoplastic character was revealed. Transurethral resection of the lesion (TURB) was performed. Histopathology revealed endosalpingiosis with small endocervical foci. The picture of hyperplasia met the criterion of mullerianosis. Conclusion. Bladder Mullerianosis is a very rare disease that occurs mainly in women of reproductive age. It has very good prognosis. It is important to differentiate the lesion with malignant tumor. The basis for the diagnosis is the histopathological examination of the lesion tissues taken during the surgery.
Brachial artery injury
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2020) Milik, Krzysztof; Pasternak, Grzegorz; Aebisher, David
Introduction. Arterial damage associated with musculoskeletal injuries at room conditions is very common. Aim. It is worth remembering that examining a patient who has suffered an accident with a broken bone or traumatic joint dislocation may be accompanied by vascular damage. A dislocation or fracture often masks damage to the artery. Description of the case. In this paper, a 20-year-old patient being a passenger suffered upper right limb trauma in a car accident. Conclusion. The effects of vascular injuries appear only after a few hours after setting the fracture. It is not known then whether the artery was damaged at the same time with the fracture, whether the injury occurred during the adjustment, or whether the ischemia is finally the result of the pressure exerted by too tight plaster casts.
Chemiluminescence-driven Dye Excitation for Dark Photodynamic Therapy
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2017) Ożóg, Łukasz; Tabarkiewicz, Jacek; Aebisher, David
Photodynamic therapy is a treatment that uses a combination of light-absorbing photosensitizers and dissolved oxygen to kill cancer. One specific limitation of photodynamic therapy is that the visible light used for photosensitizer excitation has a short tissue penetration depth of several millimeters. This limits the application of photodynamic therapy to surface cancers in the absence of a technique to illuminate deeper tissue. Efforts to extend tissue depth to which photodynamic therapy can be applied have been attempted with use of up-conversion and persistent-luminescent nanoparticles that absorb near infrared light and emit visible light for photosensitizer excitation, yet an initial excitation with an external light source is still required. More recently, systems employing chemiluminescence as an excitation energy source designed to bypass the use of external light have been developed and investigated as potential agents that could overcome the problem of achieving photodynamic therapy in deep tissue. We wish to provide an overview of several systems that have been recently reported that employ both radiative and non-radiative chemiluminescent energy transfer for photosensitizer excitation that have been developed in the hope of achieving “dark” photodynamic therapy. This article reviews several of these important new developments in the design of photodynamic therapeutic systems that utilize chemiluminescence.
Clinical application of advanced neuroimaging techniques – Magnetic Resonance Spectroscopy
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2017) Guz, Wiesław; Bober, Zuzanna; Ożóg, Łukasz; Truszkiewicz, Adrian; Przypek, Aneta; Aebisher, David; Bartusik-Aebisher, Dorota; Urbanik, Andrzej
Continuous scientific research and the increasing saturation of the medical market in Poland implements the possibilities of using advanced MR techniques including MRS in everyday practice. This method, which has so far been used primarily for research purposes, can bring measurable benefits to patients not only in terms of clarifying diagnosis and narrowing differential diagnosis, but also monitoring the course of various diseases and their treatment. Here we present the basic principles of performing and interpreting spectroscopic spectra and possible clinical applications and development prospects of MRS. The literature reviewed both Polish and foreign articles both historically and in the past 10 years. The paper presents methodological issues related to the proper performance of magnetic resonance spectroscopy (MRS) and spectral composition and the role of major metabolites, as well as current clinical applications and directions of MRS development.
Coagulation markers in diagnostic and monitoring of thromboembolic complication in COVID-19
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Czarnecka-Czapczyńska, Magdalena; Bartusik-Aebisher, Dorota; Krupka-Olek, Magdalena; Aebisher, David; Cieślar, Grzegorz; Latos, Wojciech; Kawczyk-Krupka, Aleksandra
Introduction. Coronavirus disease 2019 (COVID-19) was first observed in China in Wuhan city, Hubei province in December, 2019, and specified as a pandemic by the World Health Organization (WHO). COVID-19 is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARSCoV2). Aim. The aim of this article is to discuss epidemiology of thromboembolic complication in COVID-19. Material and methods. This article is a review done in regards to discuss clinical features of the anticoagulation treatment in COVID-19. Analysis of the literature. A review is discussed an anticoagulation treatment in 41 manuscripts. Conclusion. Most commonly coagulation abnormalities in patient with COVID-19 is mild thrombocytopenia. Apart from their typical role in thrombosis and hemostasis, platelets mediate key aspects of immune and inflammatory.
Crohn’s disease – a case study
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Pasternak, Grzegorz; Bartusik-Aebisher, Dorota; Aebisher, David; Filip, Rafał
Introduction. Surgical treatment of patients with Crohn’s disease can be a big challenge, due to the high risk of complications that is associated with chronic inflammatory process, immunosuppressive, biological treatment, malnutrition, patient’s wasting or prone to recurrence of inflammatory changes. Aim. The aim of this work ist o present the case of patient underwent surgery, resection of the terminal ileum, right hemicolectomy and segmental jejunum resection. Description of the case. In this case patients with Crohn’s disease were accompanied by progressive nutritional deficiencies and cachexia. Conclusion. Crohn’s disease can lead to very severe abdominal and septic complications that require long-term treatment, repeated surgery, and open belly therapy with the use of vacuum therapy.
Crohn’s disease complicated with a bladder-fistula – a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Pasternak, Grzegorz; Bartusik-Aebisher, Dorota; Aebisher, David; Filip, Rafał
Introduction. Entero-bladder fistula (fistula entero-vesicalis) is a pathological connection between the lumen of the gastrointestinal tract and the bladder. Entero-bladder fistulas are not a common condition. The main reason for the formation of entero- bladder fistulas are intestinal diseases occurring within the intestinal loop adjacent to the bladder resulting in the formation of an abnormal channel, the connection between the above structures Aim. The aim is to present the causes of the fistulas can be divided into congenital and acquired (intestinal infection, cancer, Crohn’s disease, resulting from trauma and iatrogenic). Clinical manifestations of the biliary-bullous fistulae may be from the digestive or urinary tract. The most characteristic ailments are pneumaturia, fecuria, urge to urinate, frequent urination, lower abdominal pain, hematuria, urinary tract infection. Description of the case. The article discusses the case of a patient with Leśniowski-Crohn disease complicated with a bladder- fistula. The treatment of entero-bladder fistulas is primarily surgical, it consists in resection of the fistula together with resection of the affected intestine and bladder wall fragment. Conclusion. The test confirming the presence of an entero-bladder fistula is a test with oral administration of poppies, although it happens that the test result may be negative, especially in the case of a bladder-follicular fistula. Among the tests useful in the diagnosis of entero-bladder fistula include abdominal ultrasound, computed tomography, magnetic resonance imaging, endoscopic tests (colonoscopy or cystoscopy).
Cytisine parameters measured in the 1.5 Tesla magnetic field
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2020) Bober, Zuzanna; Galiniak, Sabina; Leksa, Dawid; Dynarowicz, Klaudia; Aebisher, David; Ostańska, Elżbieta; Czmil, Anna; Bar, Piotr; Bartusik-Aebisher, Dorota; Tutka, Piotr
Introduction. Cytisine, Cytisinum (C11H14N2O), is an organic chemical compound. Cytisine is heterotricyclic compound that is the toxic principle in Laburnum seeds and is found in many members of the Fabaceae (legume, pea or bean) family. Aim. The aim of the study is to measure the influence of water on the form of drug in the magnetic field 1.5 Tesla. Material and methods. For this purpose, magnetic resonance imaging tests were performed to check the solubility of pure cytisine, Desmoxan tablets and Tabex capsules. Results. From a pharmacological point of view, both Desmoxan tablets and Tabex capsules should exert the same effect on the human body, this is due to the identical content of the active substance, in this case cytisine (1.5 mg). Conclusion. The differences in the results obtained may be related to additional excipients that contain medications, but it is believed that they should not have a negative impact on the action of the active substance.
Diagnostic and therapeutic difficulties of tick-borne encephalitis – a two case reports
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2020) Przetacznik, Dominika; Leksa, Natalia; Aebisher, David; Galiniak, Sabina; Bartosz, Seweryn; Leksa, Dawid; Bartusik-Aebisher, Dorota
Introduction. The paper presents epidemiology, routes of infection, forms of the disease, diagnostic and treatment methods, and prophylaxis of tick-borne encephalitis. Aim. In this paper, we present two descriptions of the cases of tick-borne encephalitis. Description of the cases. Case 1. A 60-year-old man with fever up to 39 degrees for 3 days, multi-site headache and other body aches, as well as an earstuck feeling. The day before hospitalization, there was a feeling of numbness on the right side of the face from eye level to the chin and speech distortion. Case 2. A 60-year-old patient with headaches and an increase in temperature to 39 degrees for 3 days, who, approximately, three weeks earlier was ticked by a tick in the lower parts of the back. Conclusion. It is also important for doctors to take a broader view and to make society aware of that Lyme disease is not associated only with Lyme disease. Further work is also needed towards effective treatments for Tick-borne encephalitis (TBE).
Effect of photodynamic therapy with hypericin on the secretion of selected cytokines of colorectal cancer cells tested in vitro
(Publishing Office of the University of Rzeszow, 2023-06) Międzybrodzka, Anna; Kawczyk-Krupka, Aleksandra; Aebisher, David; Cieślar, Grzegorz; Czuba, Zenon Paweł
Introduction and aim. Photodynamic therapy is a complex process involving the introduction of photosensitizers into the patient’s body and irradiation of them in order to destroy the lesion, and activate the immune system. An important role in photodynamic therapy is played by photobiochemical and physical mechanisms that affect the tumor vessels and lead to the death of the damaged cell. The aim of the study is to determine the effect of photodynamic therapy with the use of Hypericin (Hyp) on the secretion of selected cytokines by colorectal cancer cells. Material and methods. Two colorectal cancer cell lines SW480 and SW620 were used in the study. Cells treated Hypericin were exposed to visible light. Then cell viability was determined by the MTT assay with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium. Assays were performed for control samples without hypericin and light exposure, with Hyp without light exposure, without Hyp and irradiated with light, and test samples with Hyp and light exposure. Results. In the experiment we reveal, that Hyp- photodynamic activity does not influence the secretion of cytokines. Conclusion. The obtaining results confirming the destructive effect of Hyp- PDT on the colon cancer cells, show a possibility of extending the indication for photodynamic therapy using Hyp, qualification of precancerous changes.
Effects of hypericin-mediated photodynamic therapy on GM-CSF, MIF, VCAM-1 and ICAM-1 secretion in colorectal cancer cells in vitro
(Publishing Office of the University of Rzeszow, 2023-06) Międzybrodzka, Anna; Kawczyk-Krupka, Aleksandra; Aebisher, David; Cieślar, Grzegorz; Czuba, Zenon Paweł
Introduction and aim. Photodynamic therapy with hypericin (HYP-PDT) is gaining importance as a potential treatment method for malignant neoplasms. The following study investigated whether HYP-PDT influences the secretion of certain factors of colon cancer cells in vitro. Material and methods. Two colon cancer cell lines were used in this experiment: SW480 and SW620. The cells were properly prepared and then treated with photodynamic therapy with hypericin at concentrations of 0.25 μM and 0.5 μM and irradiation at the doses of 1 J/cm 2 , 5 J/cm 2 and 10 J/cm 2 . After using HYP-PDT, changes in the concentrations of four factors: GM-CSF, MIF, VCAM-1 and ICAM-1 were analyzed in the test tubes. Results. In the case of SW480 cells: a notable decrease in GM-CSF, MIF, VCAM-1 and ICAM-1 secretion was noted after HYP-PDT. In the case of SW620 cells, after HYP-PDT: no effect on GM-CSF secretion was noted; it inhibited the secretion of VCAM-1 and MIF and increased the secretion of ICAM-1. Conclusion. Photodynamic therapy with hypericin shows immunomodulatory potential in an in vitro cell line experiments. This may indicate its possible ability to modify the course of malignant tumors and therefore requires further research.
Efficacy and safety of biosimilar IFX (CT-P13) and adalimumab in patients with active fistulizing perianal Crohn’s disease naďve to anti-TNF therapy: preliminary results from the POLIBD study
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Pękala, Anna; Aebisher, David; Pardak, Piotr; Filip, Rafał
Introduction. The development of perianal fistulas are a risk factor in colonic and rectal disease. Perianal CD treatment requires a combination of surgical and therapeutic treatments aimed to prevent septic complications, reduce fistula discharge and ultimately heal fistulas. Aim. The purpose of the study was to evaluate the efficacy and safety of biosimilar IFX (CT-P13) and adalimumab in active fistulizing perianal Crohn’s disease (CD) in patients from the Subcarpathian Region (South-Eastern Poland). Material and methods. Thirty patients with CD with perianal fistulas naive to anti-TNF therapy were enrolled (13 females/ 17males) ranging from 18 to 64 years of age. Twenty-one were treated with biosimilar infliximab (CT-P13), nine were treated with adalimumab (ADA). The treated patients had ileal CD (4), ileo-colonic CD (13) or colonic CD (13). All of them received standard immunosuppression with no additional steroid therapy. Response was evaluated at week 16 and 40 after the first CT-P13 dose, and 16 and 40 weeks after the first ADA dose. Remission was defined as the complete closure of all fistulas and partial response as a reduction (≥50%) in the number of draining fistulas. Results. Treatment outcomes with CT-P13 and ADA were both effective and similar in the percentage of patients with perianal fistula improvement, perianal fistula remission, no effect or observed adverse events. Conclusion. In patients with active fistulizing CD, both CT-P13 and ADA were effective and safe, however a slight superiority of CT-P13 was visible.