Methods of singlet oxygen generation and detection for understanding photodynamic processes

Abstract

Introduction. Photodynamic therapy (PDT) is a clinically approved therapeutic procedure that exerts selective cytotoxic activity toward malignant cells. Aim. Our goal is to present the PDT procedure which involves administration of a photosensitizing agent followed by irradiation at a wavelength corresponding to the absorbance band of the photosensitizer and energy transfer to ground state oxygen to generate cytotoxic singlet oxygen Material and methods. Analysis of literature. Results. In this paper we described the basics of PDT and lifetime of singlet oxygen in different media.