European Journal of Clinical and Experimental Medicine T.17, z. 1 (2019)

URI dla tej Kolekcjihttp://repozytorium.ur.edu.pl/handle/item/4512

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Cardiopulmonary exercise test performed on a football player: a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Wołoszyn, Filip
Introduction. Cardiopulmonary Exercise Test (CPET) can precisely determine aerobic capacity, conjugate and independent functions of the pulmonary cardiovascular, and skeletal muscle systems. Aim. To describe CPET feedback from a high stamina professional football player. Description of the case. The test took place in course of one CPET session. The method of an individual case study was used in this research. The participant was a local team football player. According to the coaches’ opinion, this player had the best ability to perform long distance work. The test was performed using a cycle ergometer. Cardiopulmonary Exercise Test was performed with a cycle ergometer RAMP test. Conclusion. The player’s capacity is at a level that allows us to outline his results as a unique case.
Efficacy and safety of biosimilar IFX (CT-P13) and adalimumab in patients with active fistulizing perianal Crohn’s disease naďve to anti-TNF therapy: preliminary results from the POLIBD study
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Pękala, Anna; Aebisher, David; Pardak, Piotr; Filip, Rafał
Introduction. The development of perianal fistulas are a risk factor in colonic and rectal disease. Perianal CD treatment requires a combination of surgical and therapeutic treatments aimed to prevent septic complications, reduce fistula discharge and ultimately heal fistulas. Aim. The purpose of the study was to evaluate the efficacy and safety of biosimilar IFX (CT-P13) and adalimumab in active fistulizing perianal Crohn’s disease (CD) in patients from the Subcarpathian Region (South-Eastern Poland). Material and methods. Thirty patients with CD with perianal fistulas naive to anti-TNF therapy were enrolled (13 females/ 17males) ranging from 18 to 64 years of age. Twenty-one were treated with biosimilar infliximab (CT-P13), nine were treated with adalimumab (ADA). The treated patients had ileal CD (4), ileo-colonic CD (13) or colonic CD (13). All of them received standard immunosuppression with no additional steroid therapy. Response was evaluated at week 16 and 40 after the first CT-P13 dose, and 16 and 40 weeks after the first ADA dose. Remission was defined as the complete closure of all fistulas and partial response as a reduction (≥50%) in the number of draining fistulas. Results. Treatment outcomes with CT-P13 and ADA were both effective and similar in the percentage of patients with perianal fistula improvement, perianal fistula remission, no effect or observed adverse events. Conclusion. In patients with active fistulizing CD, both CT-P13 and ADA were effective and safe, however a slight superiority of CT-P13 was visible.
Expression of heat shock protein 70 in the tissue of patients with laryngeal squamous cell carcinoma
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Barańska, Aleksandra; Maziarz, Aleksandra; Roliński, Jacek; Klatka, Janusz; Dudzińska-Ćwiek, Katarzyna; Tabarkiewicz, Jacek
Introduction. Laryngeal squamous cell carcinoma (LSCC) is a common type of head and neck malignancy. Because of unsatisfactory results of therapy, development of new strategies for LSCC treatment is needed. It is believed that heat shock protein 70 (HSP70) is involved in pathogenesis of LSCC. Thus, targeting HSP70 seems to be promising strategy for laryngeal cancer treatment. Aim. The aim of the study was to assess the HSP70 concentration in laryngeal squamous cell carcinoma specimens and its correlation with tumor volume and TNM staging. Material and methods. An ELISA method and a Bradford protein assay were used to evaluate the HSP70 concentration in peripheral blood cells, tumor tissue and lymph nodes from the patients suffering from LSCC. Results. We demonstrated that the HSP70 concentration is significantly different between examined compartments. The highest level was observed in peripheral blood, while the lowest was in the lymph nodes. The HSP70 expression was correlated to tumor volume. Conclusion. Our results showed varied expression of HSP70 in tissue from patients with LSCC, but there was no association between HSP70 concentration and TNM staging. Currently, application of HSP70 inhibition as a LSCC treatment could be rather associated with systemic blocking of this molecule than target inhibition in tumor tissue. However, further analysis on a larger group of patients is needed.
Extraction of asymptomatic impacted third molars – a review
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Błochowiak, Katarzyna; Aleksandrzak, Paweł; Kropielnicki, Kacper; Handschuh, Jakub; Pawlik, Patrycja; Stanek, Anna
Introduction. The prophylactic extraction of asymptomatic wisdom teeth is defined as the surgical removal of wisdom teeth in the absence of local disease. Early extraction of asymptomatic third molars is considered beneficial to patients to prevent the risk of future pathology, and to minimize operative and postoperative risks. The second concept is watchful monitoring of asymptomatic wisdom teeth, adhering to specific indicators for their extraction. Aim. The aim of this paper is to present and evaluate the indications and effects of prophylactic extraction of asymptomatic impacted third molars in adolescents and adults, compared with their retention and watchful monitoring. Material and methods. This study is based on analysis of literature. Conclusion. There exist clear indications for the extraction of third molars which are associated with pathology. Prophylactic extractions of asymptomatic impacted third molars should be performed only before 20 years of age. In older age, asymptomatic third molars should be retained and watchfully monitored, and removed only in cases of evident clinical or radiological symptoms.
Genetic risk factors of Alzheimer’s disease
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Skrzypa, Marzena; Potocka, Natalia; Bartosik-Psujek, Halina; Zawlik, Izabela
Introduction. Alzheimer’s disease (AD) is one of the most common neurodegenerative diseases, which is a serious health problem for societies that live longer. Spontaneous dominant mutations and polymorphisms of selected genes play an important role in development of AD. Aim. Several polymorphisms in selected genes strongly associated with development of Alzheimer’s disease were highlighted in this review: APOE, CYP46, APP, PSEN1, PSEN2, UBQLN1, BACE1, PRND, APBB2, TOMM 40. These gene polymorphisms have a significant role in the development of Alzheimer’s disease and they have potential to be biomarkers. Researchers combine efforts to find significant polymorphisms that would ensure that a person is predisposed to the occurrence of disease symptoms. This topic is often taken up by scientists seeking to develop effective genetic tests for diagnosing AD. Material and methods. Analysis of literature from web of knowledge: Web of Science (all database), NCBI and PubMed. Results. We reviewed the selected important genes and polymorphisms which are most often associated with development of AD. Conclusion. It should be noted that nowadays scientists strive not to focus on only one polymorphism in the gene but on several polymorphisms in different genes concomitantly and above all on interactions between them to the diagnosis of this disease. Only this approach to AD will contribute to the creation of appropriate identification methods. Moreover, we should use the new generation tools - the platform for collecting data and personalized medicine.
Infliximab in therapy of inflammatory bowels diseases
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Bar, Piotr; Galiniak, Sabina; Bartusik-Aebisher, Dorota; Filip, Rafał; Aebisher, David
Introduction. Infliximab is a monoclonal antibody that acts against tumor necrosis factor TNF-α. The drug is used in the treatment of autoimmune diseases. Aim. This article reviewed the efficacy and safety of infliximab for the treatment in severe ulcerative colitis. This review included studies that evaluated the clinical use of infliximab. Material and methods. This meta-analysis was performed according to systematic literature search of three major bibliographic databases (Scopus, PubMed, and Cochran). Results. Infliximab has been approved by the US Food and Drug Administration (FDA) as a medicine to treat Leśniowski and Crohn’s disease, ulcerative colitis, psoriasis, psoriatic arthritis, ankylosing spondylitis, and rheumatoid arthritis. However, further trials are required to compare other parameters of efficacy such as the clinical response with infliximab. Conclusion. In patients suffering from Crohn’s disease or ulcerative colitis under infliximab maintenance therapy, sustained good trough levels are associated with: better response and remission rates, more mucosal healing and less loss of response.
Infliximab MRI relaxation time in solution
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Bober, Zuzanna; Bar, Piotr; Pasternak, Grzegorz; Galiniak, Sabina; Bartusik-Aebisher, Dorota; Filip, Rafał; Aebisher, David
Introduction. The use of Quantitative Magnetic Resonance Imaging to drug monitoring in vitro or in vivo can provides a powerful means to map the effects of drugs on tissue activity. Aim. The purpose of this study was to measure relaxation time of antibody phantom. For this purpose, infliximab sample was used. Material and methods. The selected methods to detect relaxation time of antibody was Magnetic Resonance Imaging technique. We detected spin-lattice (T1) relaxation time and discuss differences where compare to water. Results. The measurements of spin-lattice (T1) relaxation time showed significant differences. The results obtained in phantom indicate that we can use this result for measurements of relaxation time in vitro. Conclusion. Infliximab is approved for severe cases of rheumatoid arthritis, together with methotrexate, for pronounced psoriasis and psoriasis-arthritis, ankylosing spondylitis as well as for chronic inflammatory bowel disease. We conclude, that Quantitative Magnetic Resonance Imaging can be used to monitor drug effects.
Is valgus foot always flat? The longitudinal arch of the foot and hindfoot valgus in 10–12 year-olds
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Jankowicz-Szymańska, Agnieszka; Wódka, Katarzyna; Mikołajczyk, Edyta
Introduction. Flat foot is still a controversial topic. As of yet, there are no unified definitions of this deformation. Aim. To assess the correlation between the longitudinal arch of the foot and hindfoot valgus in school-age children. Material and methods. A total of 362 pupils, 183 girls aged 10.96 ± 0.78 yrs and 179 boys aged 10.89 ± 0.78 yrs, participated in the study. Their height and weight were measured and their Body Mass Index (BMI) weight status was categorized. The Arch Index (AI) was used to assess the longitudinal arch of the foot. Hindfoot valgus was measured by a goniometer and defined as the angular deviation between the tibial anatomical axis and the calcaneus longitudinal axis. Results. About 1/3 of the participants had hollow feet and about a fifth of them had flat feet. No correlations between the longitudinal arch of the foot and the hindfoot valgus were discovered; however, a correlation between excessive weight and the longitudinal flat foot was revealed. No correlations between BMI and hindfoot malalignment were found. Conclusion. Hindfoot valgus was prevalent in a considerable proportion of boys and girls with the flat, normal and hollow foot. Therapeutic correction of valgus feet should be varied and should depend on the quality of the longitudinal arch of the foot.
Optimization of detection of circulating tumor cells by flow cytometry and qRT-PCR
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Pogoda, Katarzyna; Rybojad, Paweł; Álvarez, Inés Ramírez; Mohanathas, Thusyanthan; Hosseini, Sarah; Tabarkiewicz, Jacek
Introduction. Treatment and diagnostic process in solid tumors like lung cancer are still based on invasive methods such as bronchoscopy, solid biopsy et cetera. One of the less invasive methods is a proposed “liquid biopsy” which is based on capturing of tumor cells circulating in the blood. Aim. The aim of the study was to standardize conditions and to assess the sensitivity of the identification of circulating tumor cells (CTCs) with the use of flow cytometry and qRT-PCR. Material and methods. In the first model of CTCs, cells from the A549 lung cancer cell line were suspended in 1 ml of healthy donors’ blood in 5 spikes increasingly: 0, 10, 50, 100 and 200 and the cells were detected in flow cytometer. In the second model, cells from the A549 and H1975 lung cancer cell lines were used. Spikes were prepared as in the first model, but cells were suspended in 400 μl of healthy donors’ blood and were detected with the use of qRT-PCR. Results. An increasing number of detected cytokeratin positive events from the 1st spike “0” to the last one - “200” was observed by flow cytometry. Median value in the negative control was 0 false positive cells. In tubes from “10” to “200” the median was 5, 43.5, 58 and 78, respectively. Mean sensitivity of flow cytometry was 63.79%. In qRT-PCR, correlation between increasing number of sorted cells in several spikes and the level of mRNA expression for KRT19 gene was not observed. Conclusion. Commonly available methods like flow cytometry and qRT-PCR seem to be attractive solutions for CTCs detection, but they need pre-enrichment procedures and standardization.
Personality correlates in the tendency to incur debt in patients with and without osteoarthritis
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Łukasiewicz, Jacek; Stencel, Marcin; Raczkowski, Adam; Cwynar, Andrzej; Cwynar, Wiktor; Saran, Tomasz; Mazur, Anna
Introduction. Osteoarthritis is the most common disease of the musculoskeletal system. Osteoarthritis progresses slowly, but with time it results in movement disability and chronic pain. Its progression is also significantly associated with personality functioning and the ability to make rational life choices regarding various aspects of human life, including the economic sphere. Aim. The aim of the research was to determine how personality correlates with a tendency towards indebtedness between patients with and without osteoarthritis. Material and methods. The researched sample consisted of two groups - 50 patients diagnosed with osteoarthritis hospitalized in the Department of General and Neuro Rehabilitation in the Institute of Rural Health in Lublin and 50 healthy people. The KOS-B Questionnaire, the IVE Questionnaire, the SES Scale, the SPP-25 Scale, the Delta Questionnaire and the APSZ questionnaire were used in the research. Results. In the group of patients with osteoarthritis, the leading correlates of indebtedness are: assessment of the stress situation related to the current economic situation seen as a threat, and perceiving it as harm or loss. In the healthy group, the leading correlates of incurring financial liabilities are impulsiveness, self-esteem, assessment of the stress situation related to the current economic situation seen as a threat, openness to new experiences and sense of humor, empathy, and the need for social approval. Conclusion. In the group of patients with osteoarthritis, the factor conducive to incurring financial liabilities is the assessment of the stressful situation related to the current economic situation seen as a threat, while perceiving it as harm or loss leads to abandoning reliance on credit. In the healthy group, the factors that favor this type of activity are impulsiveness, self-esteem, the assessment of the stress situation related to the current economic situation seen as a threat and the need for social approval; the factors protecting against indebtedness in healthy persons are openness to new experiences and sense of humor as well as empathy.
Polish linguistic adaptation of the Western Ontario Shoulder Instability Index
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Bejer, Agnieszka; Mastej, Sabina; Domka-Jopek, Elżbieta; Probachta, Mirosław; Kulczyk, Marek; Griffin, Sharon
Introduction. The Western Ontario Shoulder Instability Index (WOSI) is a patient reported outcome measure to assess the impact of unstable shoulder on various spheres of a patient’s life. The aim of this paper is to present the stages of linguistic adaptation of the English version of the WOSI questionnaire into the Polish version (WOSI-PL). Material and methods. The research used the procedure of translation of research tools recommended by Mapi Research Institute, which allows to minimize errors resulting from the translation. Results. Two versions of the translation were created: A1V and A2V, and based on them, a common version BV was agreed. An English native speaker made a back translation version – BTV. Next, the BTV was compared with the source version SV, corrections were made and the CV version was created. Based on the analysis of experts’ assessments, a DV version was agreed, then evaluated by a group of 6 patients. The patients’ responses was analyzed, and the final version – WOSI-PL was created. Conclusion. The WOSI-PL constitutes a valuable tool to evaluate health-related quality of life in patients with shoulder instability. The questionnaire before being introduced to scientific research and clinical practice will be subjected to a validation process in order to evaluate psychometric properties.
Rapidly progressing dementia as a manifestation of the Creutzfeldt-Jakob disease: an analysis of two cases
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Bartosz, Seweryn; Leksa, Natalia; Uberman-Kluz, Dominika; Szymczak, Artur; Biesiadecki, Marek; Galiniak, Sabina
Introduction. Creutzfeldt-Jakob disease (CJD) is a rare and fatal neurodegenerative disease of the central nervous system which is caused by an infectious protein called prion. Multiple forms of CJD have been classified including sporadic (more than 90% cases), familial, iatrogenic and variant type of disease. CJD, especially in its early stages, is a highly challenging illness to diagnose. Aim. Article aims to present cases of Creutzfeldt-Jackob disease with early symptoms of rapidly progressing dementia at the initial stage of CJD. Description of the cases. This paper describes two cases of patients with suspected CJD with a history of rapidly progressive dementia admitted to the Department of Neurology, MSWiA Hospital in Rzeszów. Conclusion. Despite the fact that CJD is an incurable illness and there is no cure guaranteeing recovery, it is important to make the right diagnosis. Assay of 14-3-3 protein in cerebrospinal fluid is a sensitive and specific marker which is helpful in the diagnosis of CJD. The only relevant method of correctly confirming a diagnosis of this disease is by performing a brain biopsy.
Safety and efficacy of vaccinations in patients from high-risk groups: new challenges in the era of vaccine hesitancy
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Czajka, Hanna
Introduction. Vaccinations are one of the most effective medical interventions that protect people against infectious diseases. It should be noted that a new vaccine licensing is always preceded by clinical trials assessing its safety and efficacy. Anti-vaccine propaganda carried out by vaccination opponents has become an international problem with a global reach. Aim. To review the literature on vaccinations of patients from high-risk groups. Material and methods. A literature review of the following databases has been conducted: EBSCO, PubMed, Science Direct, and Springer Link. Results. High-risk groups in the paediatric population include pre-term born infants, patients after stem cell transplantations, children with allergies and other chronic diseases. Vaccinations in the examined groups are generally safe and are an effective method of preventing infections. Conclusion. At a time when the level of vaccine skepticism is high and the epidemiological situation of many diseases is unstable, patients who are more susceptible to infection are particularly endangered. High level of knowledge of health care professionals and their personal positive attitude towards vaccinations are important for improving the vaccination coverage rates. In the light of measles epidemic outbreaks and an almost geometric increase in the number of pertussis cases noted recently, actions are needed to achieve herd immunity.
Selected factors influencing physical fitness in the elderly
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Wybraniec, Agnieszka; Boguszewski, Miłosz; Nizio, Norbert; Wyszyńska, Justyna; Drzał-Grabiec, Justyna
Introduction. In effect of the ageing process, there are involutional changes in older adults both in the mental and in the physical spheres. Some factors may slow down the ageing process. Physical activity is one of these factors. Physical ability is one of the most important indicators of quality of life of older adults, and it can be shaped by physical activity. Aim. The aim of the study was to assess chosen factors that influence physical ability of adults older than 65 years. Material and methods. The study involved 63 older adults. The criterion for inclusion in the study was the age - older than 65 years. There were 37 women and 26 men in the study population. All the subjects were able to walk without assistance, and they had no contraindications to do functional tests. We used a questionnaire of our own design, three SPPB trials, Up&Go test; we calculated the BMI indices and assessed depression scale. Results. We found that older adults rarely engage in physical activity. The low level of physical activity of the older adults was reflected in equally low physical ability level, as assessed by functional tests. We found a directly proportional dependence between body mass and the level of functional limitations and risk of falls. Conclusion. In our functional tests, we found a statistically significant correlation with regard to age and BMI index.
The advancement of imaging in diagnosis of prostate cancer
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Osuchowski, Michał; Aebisher, David; Gustalik, Joanna; Bartusik-Aebisher, Dorota; Kaznowska, Ewa
Introduction. Multiple imaging methods have been used to stage prostate carcinoma. Some of them are easily accessible, others very accurate. The advancements over many years have been taken under consideration and now every imaging method has a specific role in the diagnosis of this malignancy. Aim. There are over 1,100,000 cases of prostate carcinoma diagnosed every year around the world. Imaging examinations have to be introduced to accurately stage, and therefore properly treat this disease. This review concentrates on advantages and disadvantages of different imaging methods. Material and methods. The literature search was performed. Results. Imaging methods serve specific goals. TRUS is recommended for acquiring biopsy specimen due to high accessibility and low cost of the examination. Conclusion. The best tool for staging prostate carcinoma and finding suspicious lesions when attempting second biopsy is mpMRI or bpMRI.
The epidemiological pattern of oroantral communication – a retrospective study
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Pawlik, Patrycja; Stanek, Anna; Wyganowska-Świątkowska, Marzena; Błochowiak, Katarzyna
Introduction. Oroantral communication (OAC) between the maxillary sinus and the oral cavity is an infrequent post-surgical complication occurring most commonly after extraction of posterior maxillary teeth. Aim. To present the characteristics of OAC and predisposing factors as well as evaluate postoperative pharmacological therapy and complications in patients with an OAC. Material and methods. In this retrospective study, medical records of 63 patients with diagnosed OAC between 2011 and 2018 were analyzed. Results. The most frequent causes for tooth extraction leading to an OAC were periodontitis (n=34; 54%), carious destruction of the tooth (n=14; 22.2%), and tooth impaction (n=10; 16%). First molars (n=28; 44.4%), second molars (n=14; 22.2%) and third molars (n= 13; 20.6%) were the most frequently related teeth to OACs. The majority of OACs appeared in the fourth (n=22; 35%) and third (n=20; 31.7%) decades of life. Conclusion. OACs are rarely seen on an everyday basis by general practitioners; however, if left untreated, they may lead to further serious complications. Proper postoperative precautions must be taken in order to prevent further complications, and thus the evaluation of predisposing factors is of great importance.
Upper limb analysis measured by inertial measurement unit tool: a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2019) Wołoszyn, Filip; Czmil, Anna
Introduction. This article reports differences in accurate and inaccurate forehand and backhand strokes in tennis. The tests were carried out on a professional tennis player. The duration of a stroke, the heights of the individual segments of the right upper limb and differences in the heights of the segments at the beginning and at the end of every phase of stroke were examined. Aim. The major aim of the work was to expose upper limb disparity in stokes. Description of the case report. The research tool was inertial motion sensors (IMS) based on an accelerometer, a gyroscope and a magnetometer. A professional tennis player was examined using the individual case method and kinematic analysis. Results. The analysis concerned the average time to perform forehand and backhand strokes during all phases of the stroke, i.e. preparation, acceleration and follow-through phases. The average heights of the individual upper limb segments during a stroke were also taken into account. The results of the study are meant to show how the movement of the upper limb affects the accuracy and velocity of a stroke. Conclusion. The movements of individual segments in some accurate strokes were similar to those in inaccurate strokes.

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