European Journal of Clinical and Experimental Medicine T.19, z. 1 (2021)

URI dla tej Kolekcjihttp://repozytorium.ur.edu.pl/handle/item/6115

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Serum levels of anti-corona virus specific -IgG and -IgM antibodies in COVID-19 patients at admission and at discharge
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Ganiyu, Arinola
Introduction. Clear understanding of duration of antibody based protective immunity following natural infection with SARSCoV- 2 will give idea about the efficacy of proposed prophylactic vaccines against SARS-CoV-2, establishment of herd immunity and use of convalescent plasma. Aim. This study clarified the kinetics and magnitude of the initial antibody response against SARS-CoV-2 in a cohort of symptomatic COVID-19 patients from Ibadan, Nigeria. Material and methods. This study quantified immunoglobulin M (IgM) and G (IgG) antibodies recognizing the SARS-CoV-2 Spike (S) protein in 35 symptomatic COVID-19 patients at admission and at discharge using ELISA. Results. CovIgG was positive in none (0)% and 20% of COVID-19 patients at admission and at discharge respectively while CovIgM was positive in 54% and 69% of COVID-19 patients at admission and at discharged respectively. The level of CovIgG was significantly higher in COVID-19 patients at discharge compared with the level at admission while the level of CovIgM was insignificantly reduced in COVID-19 patients at discharge compared with the level at admission. Conclusion. The data indicates increased anti-SARS-COV-2 IgG Spike antibody in symptomatic COVID-19 at discharge, thus providing basis for antibody-based therapies to treat COVID-19 patients.
Anxiety states and knowledge of COVID-19 among pregnant women during the pandemic in Turkey – a cross-sectional study
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Hoşoğlu, Esra; Şahin, Berkan; Önal, Bedia Sultan; Baki Yıldırım, Sema
Introduction. Infectious outbreaks have negative effects not only on the physical health of the society but also on the mental health. Aim. To evaluate the anxiety states and knowledge of COVID-19 during the pandemic in pregnant women. Material and methods. Cross-sectional study conducted in a university hospital in Turkey. A total of 199 pregnant women were included in the study. The State-Trait Anxiety Inventory (STAI), two questionnaires to evaluate the knowledge about COVID-19, and COVID-19-related anxiety were applied to all the women. Results. The highest level of COVID-19-related anxieties were about their spouses or newborns contracting COVID-19, effects of drugs on fetus and contracting COVID-19 during delivery. There was a negative correlation between gestational week and the questionnaire of COVID-19-related anxieties (r=-0.152, p=0.037). STAI total score was 76.48±14.11, and STAI-T scores (42.39±7.66) were higher than STAI-S scores (34.09±8.77). Although their general knowledge about the disease was relatively good, their level of knowledge on issues that pertained specifically to pregnancy was low. Conclusion. These findings indicated more than four months had passed since the pandemic came to the country but, pregnant women were very worried and did not have enough information about the disease.
The expression of CD44, CD90 and CD133 in response to cisplatin in hepatocellular cancer cells
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Donmez Cakıl, Yaprak; Ozunal, Zeynep Gunes; Kayalı, Damla Gokceoglu; Aktas, Ranan Gulhan
Introduction. Cancer is a leading cause of mortality. Hepatocellular cancer is one of the malignancies associated with poor outcome and resistance to pharmacotherapy. Cancer stem cells (CSCs) contribute to resistance to therapy and hence lead to the treatment failure of tumors. Aim. This study aims to explore the expression of CSCs in response to cisplatin treatment in HepG2 hepatocellular cancer cell line. Material and methods. Cell proliferation test, CCK-8, was used to evaluate the cell proliferation following cisplatin treatment for 72 hours. The expressions of CSC markers CD44, CD90, and CD133 were assessed by flow cytometric analysis. Results. The results showed a dose-dependent decrease in cell proliferation and increased expression of CSC markers CD44 and CD90 in response to cisplatin. Conclusion. Understanding the roles of CSC markers may point to new targets and therapeutic strategies to predict and overcome cisplatin resistance.
Histological changes following the administration of two different chondroitin sulfate products in experimental osteoarthritis models in rats
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Nosivets, Dmitriy; Montell, Eulalia; Opryshko, Valentine
Introduction. Osteoarthritis (OA) is generally a progressive disease that affects synovial joints, resulting in abnormalities to articular cartilage subchondral bone, synovium, and adjacent soft tissues. Aim. The purpose of this work was to examine the histological changes in knee cartilage and bone following the administration of two different chondroitin sulfate products in two experimental OA models in rats. Material and methods. OA was induced in rats by either a single injection of mono-iodoacetate or four once-weekly injections of dexamethasone. 70 adult rats were included: 30 received mono-iodoacetate, 30 received dexamethasone and the 10 remaining controls received no injection. Samples of knee bone and cartilage were then analyzed histologically. Results. Animals with OA that received CS had significantly less inflammation, improved motor activity, and better analgesia compared with those that did not receive CS, with little difference between products. Histologically, both products reduced the signs of OA and resulted in the activation of regenerative processes of cartilage and bone and stimulation of proliferation and formation of amorphous material. Conclusion. These results substantiate the importance of using high-quality pharmaceutical-grade CS to ensure optimal efficacy and safety of the final product in patients with OA.
Changes in haematological parameters and serum beta-2-microglobulin levels in CD4+ T-cells-stratified Nigerian HIV patients
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Olaniyi, John Ayodele; Emeka, Godwin Joseph; Onifade, Abdulfatah Adekunle; Adeyanju, Alaruru Olusoji; Rahamon, Sheu Kadiri
Introduction. Reports have shown that there is a rise in beta-2-microglobulin (β2M) concentration in patients with HIV infection and that the degree of elevation correlates well with the extent of disease burden and could be an independent prognostic marker for death. However, there is the dearth of information on the interplay between alteration in haematological profile, a common cause of morbidity and mortality in HIV, and β2M. Aim. Changes in selected haematological parameters and β2M in Nigerian HIV patients stratified based on CD4+ T-cells counts were thus assessed in this study. Material and methods. Forty-eight asymptomatic, drug naive HIV patients were enrolled into this cross-sectional study. Haemoglobin concentration (Hb), packed cell volume (PCV), total and differential white blood cell count, platelet count and CD4+ T-cells count were determined using standard methods while serum levels of β2M were determined using ELISA. Thereafter, the patients were stratified into three groups based on the CD4+ T-cells count. Results. Hb and lymphocyte counts increased with increasing CD4+ T-cells count. In contrast, neutrophils percentage, MCV and MCH reduced with increasing CD4+ T-cells count. The mean lymphocytes percentage was significantly higher while the mean neutrophils percentage was significantly lower in patients with CD4+ T-cells count of 500–800 cells/μl compared with the patients with CD4+ T-cells count <200 cells/μl. Similarly, the mean MCV was significantly lower in patients with CD4+ T-cells count of 500–800 cells/μl compared with patients with CD4+ T-cells count of 200–499 cells/μl and patients with CD4+ T-cells count <200 cells/μl. β2M had significant positive correlation with WBC and neutrophils percentage but had a significant negative correlation with lymphocytes percentage and MCH in patients with CD4+ T-cells count <200 cells/μl. However, β2M had significant positive correlation with PCV, Hb, monocytes and morphology in patients with CD4+ T-cells count of 500–800 cells/μl. Conclusion. It could be concluded from this study that HIV infection is associated with alteration in haematological profile and the alteration is CD4+ T-cells count-dependent. Also, elevation in β2M concentration appears to be a marker of lymphopaenia in patients with low CD4+ T-cells count.
Analysis of the bacterial biofilm formation in different models of the in vitro culture
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Bogut, Agnieszka; Magryś, Agnieszka
Introduction. Microtiter plate assay (MPA) remains one of workhorses of in vitro biofilm research but it requires optimization of experimental conditions to fulfill the biofilm formation requirements of different bacterial pathogens. Aim. The aim was to determine the effect of TSB and RPMI1640 culture media and selected culture variables (O2 vs. 5% CO2, extended incubation time) on the biofilm production by bacteria commonly involved in biofilm-related infections: Enterococcus faecalis (EF), Escherichia coli (EC), Staphylococcus aureus (SA), Pseudomonas aeruginosa (PA), Klebsiella pneumoniae (KP). Material and methods. The investigation was performed using the MPA with crystal violet. Results. Statistically significant (p<0.05) increase in biofilm production between 24h and 72h time points was observed for EF (TSB o2, RPMIo2 and RPMIco2), EC (TSBo2), SA (TSBo2, TSBco2), KP (TSBo2, TSBco2), PA (RPMIco2, TSBco2). The TSB caused a significantly greater stimulation of biofilm production compared to RPM1640. It outcompeted RPMI1640 irrespective of the atmospheric conditions for SA and KP and under aerobic conditions for EF. Conclusion. Although the TSB provided the most optimal conditions for biofilm production, the process was influenced by the strain type, atmospheric conditions and period of cultivation which limits the ability to design a single universal model of the in vitro biofilm investigation.
Neonatal circumcision: profile of neonates with complications resulting from the use of plastibell
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Emeka, Chukwubuike Kevin
Introduction. Circumcision is one of the most performed surgical procedures in neonates. Aim. The aim of this study was to evaluate our experience with neonates who developed complications following the use of plastibell for circumcision. Material and methods. This was a retrospective study of male neonates who were managed for complications resulting from circumcision (performed with plastibell) over a 5-year period at the pediatric surgery unit of a teaching hospital in Enugu, Nigeria. Ethical approval was obtained from the ethics and research committee. Results. Out of the 1794 neonatal circumcisions (using plastibell) performed during the study period, 134 (7.5%) neonates had complications. Sixty percent (1074) of the circumcisions were performed in the teaching hospital while 40% were referred cases. The ages of the patients ranged from 7 to 27 days with a median of 10 days and their mean weight was 2.5 kilograms. Majority of the plastibell circumcisions that developed complications was performed by unregistered (auxiliary) nurses. Retained plastibell was the most common complication and its removal was the most performed procedure. No mortality was recorded. Conclusion. Complications following circumcision with plastibell vary widely. Retained plastibell was the most common in the present study. The most complications occurred when the circumcision was performed by auxiliary (unregistered) nurses.
Socio-economic status, iron deficiency anemia and COVID-19 disease burden – an appraisal
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Ghosh, Kusum; Chatterjee, Diptendu; Ghosh Roy, Abhisikta; Bandyopadhyay, Arup Ratan
Introduction. Severe Acute Respiratory Syndrome-2, possesses varying degrees of susceptibility and lethality worldwide and WHO declared this as a pandemic of this century. Aim. In this background, the aim of this present narrative is to provide a complementary overview of how low iron stores and mild anemia offers protection from infectious diseases like COVID-19 by restricting the viral replication and also to suggest some potential adjuvant therapeutic interventions. Material and methods. Therefore, we performed a literature search reviewing pertinent articles and documents. PubMed, Google Scholar, Chemrxiv, MedRxiv, BioRxiv, Preprints and ResearchGate were investigated. Analysis of the literature. Recent studies reported drastic systemic events taking place that contribute to the severe clinical outcomes such as decreased hemoglobin indicating anemia, hypoxia, altered iron metabolism, hypercoagulability, oxidative stress, cytokine storm, hyper-ferritinemia and thus Multi Organ Failure, reportedly hailed as the hallmark of the COVID-19 hyper- inflammatory state. Interestingly it is globally observed that, countries with higher Socio-economic status (SES) have considerably lower prevalence of Iron Deficiency Anemia (IDA) but higher Case Fatality Rate (CFR) rate due to COVID-19 while, low SES countries characterized by the higher prevalence of IDA, are less affected to COVID-19 infection and found to have less CFR, which is almost half to that of the higher SES counterpart. Conclusion. Present review presumed that,low iron stores and mild anemia may play a beneficial role in some cases by offering protection from infectious diseases as low iron restricts the viral replication.Thus, suggested iron chelation or iron sequestration as an alternative beneficial adjuvant in treating COVID-19 infection.
Membrane lipids under norm and pathology
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Marzoog, Basheer Abdullah; Vlasova, Tatyana Ivanovna
Introduction. Lipid is an essential component of the cell and its organelles membrane. The uniqueness and selectivity of lipids to specific functions and asymmetry of lipid distribution in the organelle’s membrane give the cell ability of being highly qualified and specified. Aim. The paper provides a comprehensive review of membrane lipids in different tissues and organelles of the cell in norm and disease. Material and methods. The paper analyzed the present literature data on membrane lipids behavior in physiology and pathology. Analysis of the literature. The major structural and functional lipids of the cell membrane are phosphatidylcholine > phosphatidylethanolamine. The absence/deficiency or augmentation of a specific type of lipid results in serious defects and usually life-threatening with a permanent disability. The observations discussed here suggest, the lipid peroxidation severity depends on the membrane lipid composition of the cell. Some tissue cells can handle lipoperoxidation and protect themselves from the peroxidation damaging products better, while other cells cannot compensate. Therefore, some organs are highly sensitive to peroxidation and irreversible changes occur rapidly. Conclusion. To sum up, the understanding of lipid’s role in norm and disease is clinically crucial to evaluate a novel therapeutic target to treat many metabolic disorders such as metabolic syndrome and some lysosomal storage disorders via targeting specific new signaling pathways, lipid molecules, and enzymes.
Crohn’s disease complicated with a bladder-fistula – a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Pasternak, Grzegorz; Bartusik-Aebisher, Dorota; Aebisher, David; Filip, Rafał
Introduction. Entero-bladder fistula (fistula entero-vesicalis) is a pathological connection between the lumen of the gastrointestinal tract and the bladder. Entero-bladder fistulas are not a common condition. The main reason for the formation of entero- bladder fistulas are intestinal diseases occurring within the intestinal loop adjacent to the bladder resulting in the formation of an abnormal channel, the connection between the above structures Aim. The aim is to present the causes of the fistulas can be divided into congenital and acquired (intestinal infection, cancer, Crohn’s disease, resulting from trauma and iatrogenic). Clinical manifestations of the biliary-bullous fistulae may be from the digestive or urinary tract. The most characteristic ailments are pneumaturia, fecuria, urge to urinate, frequent urination, lower abdominal pain, hematuria, urinary tract infection. Description of the case. The article discusses the case of a patient with Leśniowski-Crohn disease complicated with a bladder- fistula. The treatment of entero-bladder fistulas is primarily surgical, it consists in resection of the fistula together with resection of the affected intestine and bladder wall fragment. Conclusion. The test confirming the presence of an entero-bladder fistula is a test with oral administration of poppies, although it happens that the test result may be negative, especially in the case of a bladder-follicular fistula. Among the tests useful in the diagnosis of entero-bladder fistula include abdominal ultrasound, computed tomography, magnetic resonance imaging, endoscopic tests (colonoscopy or cystoscopy).
An aggressive locoregional orbital rhabdomyosarcoma and Li Fraumeni syndrome
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Erok, Berrin; Kıbıcı, Kenan
Introduction. Rhabdomyosarcoma (RMS) is the most common pediatric soft tissue sarcoma with 10 % of the cases occuring in the orbit. Patients often present with a rapidly developing proptosis and globe displacement. Aim. We aimed to present a very rare presentation of orbital RMS, with a giant exophytic orbital mass, a very rare presentation occuring in more advanced cases. Description of the case. A 3-year old girl presented to our hospital with a rapidly enlarging tissue like ulcerative mass. Her past medical history was remarkable with the diagnosis of embryonal rhabdomyosarcoma (RMS) and treatment with chemoradiotherapy at the age of 15 months. On magnetic resonance imaging (MRI), there was a giant heterogenously enhancing mass filling the right orbit and extending to the intracranial region. Li Fraumeni syndrome (LFS) was considered due to her sister death from neuroblastoma at an early age. Cytogenetic analysis revealed mutations of p53 gene, which supported our consideration. Conclusion. RMS is a highly malignant tumor which usually occurs sporadiacally. However, some rare syndromes are associated with increased incidence of RMS, such as LFS.
Metastasis of cancer from Merkel cells to the thyroid gland
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Przyczyna, Piotr; Trojnar, Elżbieta; Bartusik-Aebisher, Dorota; Aebisher, David
Introduction. Merkel cell carcinoma (MCC) is a rare and aggressive neuroendocrine skin cancer. Aim. Herein described is a case of hypertensive patient, after removal of Merkel cancer of the left gluteus skin (2011), after pulmonary embolism (2013), with degenerative changes of the spine and uterine myoma, chronically treated with Warfarin, because of suspected thyroid cancer. Description of the case. A 70-year-old woman case after removing Merkel cancer of the left buttock skin (2011), after pulmonary embolism (2013), with degenerative changes of the spine and uterine fibroids treated chronically with Warfarin because of suspected thyroid cancer is described. Conclusion. Increasing evidence of Merkel cell carcinoma with immunodeficiency and neoplasia, and the management and outcome of these patients requires study.
Urolithiasis due to renal dystopia and vascular anomalies
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Pliszka, Anna; Kłos, Michał; Możdżeń, Klaudia; Balawender, Krzysztof
Introduction. Variations in the urogenital vascular anomalies in the abdomen are common. However, they warrant attention due to their importance in operative, diagnostic, and endovascular procedures. Aim. The aim of this article is to show an example of a patient with rare kidney and vessels anomalies. We want to prove that those anomalies contributed to development of urolithiasis in this case. Description of the case. During dissection of abdomen in a female cadaver, unique vascular anomalies and a position disorder of both kidneys were observed. Conclusion. Vessel abnormalities were congenital and appeared simultaneously with renal dystopia. Both anomalies could contribute to stone formation and nephritis. Understanding of the urogenital anatomical variations and their relations to adjacent structures is significant during surgical and radiological procedures.
Crohn’s disease – a case study
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Pasternak, Grzegorz; Bartusik-Aebisher, Dorota; Aebisher, David; Filip, Rafał
Introduction. Surgical treatment of patients with Crohn’s disease can be a big challenge, due to the high risk of complications that is associated with chronic inflammatory process, immunosuppressive, biological treatment, malnutrition, patient’s wasting or prone to recurrence of inflammatory changes. Aim. The aim of this work ist o present the case of patient underwent surgery, resection of the terminal ileum, right hemicolectomy and segmental jejunum resection. Description of the case. In this case patients with Crohn’s disease were accompanied by progressive nutritional deficiencies and cachexia. Conclusion. Crohn’s disease can lead to very severe abdominal and septic complications that require long-term treatment, repeated surgery, and open belly therapy with the use of vacuum therapy.
Fibromatosis-like spindle-cell metaplastic carcinoma of the breast – a case report
(Wydawnictwo Uniwersytetu Rzeszowskiego, 2021) Osuchowski, Michał; Bartusik-Aebisher, Dorota; Kaznowska, Ewa; Aebisher, David
Introduction. Metaplastic breast carcinoma is expressing epithelial and/or mesenchymal tissue within the same tumor. Aim. The aim of this study is to evaluate metaplastic breast carcinoma in a case report and literature review. Description of the case. The presented case desribes metaplastic carcinoma of the breast in 65 years old female patient. Conclusion. Fibromatosis-cell metaplastic carcinoma of the breast presents a particularly large diagnostic challenge. Malignant variants of this disease have been described in the literature.

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